MHC class I diversity of olive baboons (Papio anubis) unravelled by next-generation sequencing.

Content Provider	Europe PMC
Author	van der Wiel, Marit K. H. Doxiadis, Gaby G. M. de Groot, N. Otting, N. de Groot, N. G. Poirier, N. Blancho, G. Bontrop, R. E.
Abstract	The olive baboon represents an important model system to study various aspects of human biology and health, including the origin and diversity of the major histocompatibility complex. After screening of a group of related animals for polymorphisms associated with a well-defined microsatellite marker, subsequent MHC class I typing of a selected population of 24 animals was performed on two distinct next-generation sequencing (NGS) platforms. A substantial number of 21 A and 80 B transcripts were discovered, about half of which had not been previously reported. Per animal, from one to four highly transcribed A alleles (majors) were observed, in addition to ones characterised by low transcripion levels (minors), such as members of the A*14 lineage. Furthermore, in one animal, up to 13 B alleles with differential transcription level profiles may be present. Based on segregation profiles, 16 Paan-AB haplotypes were defined. A haplotype encodes in general one or two major A and three to seven B transcripts, respectively. A further peculiarity is the presence of at least one copy of a B*02 lineage on nearly every haplotype, which indicates that B*02 represents a separate locus with probably a specialistic function. Haplotypes appear to be generated by recombination-like events, and the breakpoints map not only between the A and B regions but also within the B region itself. Therefore, the genetic makeup of the olive baboon MHC class I region appears to have been subject to a similar or even more complex expansion process than the one documented for macaque species.Electronic supplementary materialThe online version of this article (10.1007/s00251-018-1053-7) contains supplementary material, which is available to authorized users.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6006219&blobtype=pdf
ISSN	00937711
Journal	Immunogenetics
Volume Number	70
DOI	10.1007/s00251-018-1053-7
PubMed Central reference number	PMC6006219
Issue Number	7
PubMed reference number	29478145
e-ISSN	14321211
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2018-02-24
Publisher Place	Berlin/Heidelberg
Access Restriction	Open
Rights License	Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. © The Author(s) 2018
Subject Keyword	MHC Next-generation sequencing Non-human primates Haplotyping
Content Type	Text
Resource Type	Article
Subject	Genetics Immunology