Molecular Study of Quinolone Resistance Determining Regions of gyrA Gene and parC Genes in Clinical Isolates of Acintobacter baumannii Resistant to Fluoroquinolone.

Content Provider	Europe PMC
Author	Zaki, Maysaa El Sayed Abou ElKheir, Nermen Mofreh, Mohamed
Copyright Year	2018
Abstract	Introduction:Acinetobacterb aumannii (A. baumannii) is an important pathogen in health care associated infections. Quinolone resistance has emerged in this pathogen.Aims & Objectives:The aim of the present study was to determine the presence of mutations of gyrA gene and parC genes by Restriction Fragment Length Polymorphism Polymerase Chain Reaction (RFLP-PCR) among clinical isolates of A. baumanii.Materials and Methods:The study was carried out on 140 clinical isolates of A. baumannii. The isolates were subjected to molecular study of mutations of gyrA gene and parC genes by RFLP–PCR beside determination of Minimal Inhibitory Concentration (MIC) by macro dilution tube method.Results:The isolates of A. baumannii were resistant to ciprofloxacine and levofloxacin at MIC >4 µg/ml. The most isolates had MIC >128 µg/ml (42.3%). All resistant strains to ciprofloxacin of A. baumannii had mutations in gyrA and parC. The most frequent mutations were combined mutations in both genes (85.5%) and 5% had single mutation either in gyrA or parC. The most frequently combined mutations were associated with MIC >128 µg/ml (42.3%).Conclusion:From this study we can conclude that resistance to ciprofloxacin was common in clinical isolates of A. baumannii. The most frequent mutations were present in gyrA and parC. However, mutations in parC alone were not uncommon. Further large scale studies are required to elucidate the resistance pattern of A. baumannii and its molecular mechanisms.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC5958293&blobtype=pdf
Journal	The Open Microbiology Journal [Open Microbiol J]
Volume Number	12
DOI	10.2174/1874285801812010116
PubMed Central reference number	PMC5958293
PubMed reference number	29785218
e-ISSN	18742858
Language	English
Publisher	Bentham Open
Publisher Date	2018-04-30
Access Restriction	Open
Rights License	This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. © 2018 Zaki et al.
Subject Keyword	A. baumannii Quinolone resistance RFLP-PCR Molecular mechanisms Clinical isolates Mutations
Content Type	Text
Resource Type	Article
Subject	Immunology and Microbiology