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Semi-nested polymerase chain reaction-based detection of Babesia spp. in small ruminants from Northwest of Iran.
| Content Provider | Europe PMC |
|---|---|
| Author | Bazmani, Ahad Abolhooshyar, Amir Imani-Baran, Abbas Akbari, Hamid |
| Copyright Year | 2018 |
| Abstract | Aim:The present study aimed to detect Babesia ovis and Babesia motasi in the blood samples of sheep and goats from Northwest of Iran by the semi-nested polymerase chain reaction (PCR) technique.Materials and Methods:A total of 166 whole blood samples (including 123 sheep and 43 goats) were collected. In the first stage, the PCR was performed to amplify a piece of 18S rRNA gene of Babesia and Theileria genera. Then, semi-nested PCR was carried out on all PCR products to differentiate B. ovis and B. motasi.Results:The PCR indicated that totally, 19 (11.44%) out of 166 samples were positive for Babesia or Theileria spp. The semi-nested PCR showed that 38 samples (22.89%) were positive only for B. ovis. No significant association was found between the infection rate of B. ovis and age, gender and species of animals.Conclusion:In the present study, there was no evidence for B. motasi infection in small ruminants from Northwest of Iran. Therefore, B. ovis was the main causative agent of ovine Babesiosis in this region. |
| ISSN | 09728988 |
| Journal | Veterinary World |
| Volume Number | 11 |
| PubMed Central reference number | PMC5891838 |
| Issue Number | 3 |
| PubMed reference number | 29657415 |
| e-ISSN | 22310916 |
| DOI | 10.14202/vetworld.2018.268-273 |
| Language | English |
| Publisher | Veterinary World |
| Publisher Date | 2018-03-03 |
| Publisher Place | India |
| Access Restriction | Open |
| Rights License | Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. Copyright: © Bazmani, et al. |
| Subject Keyword | Babesia motasi Babesia ovis Iran semi-nested polymerase chain reaction small ruminants |
| Content Type | Text |
| Resource Type | Article |
| Subject | Veterinary |
