NDLI: Comparison of isolation platforms for detection of circulating renal cell carcinoma cells.

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Comparison of isolation platforms for detection of circulating renal cell carcinoma cells.

Content Provider	Europe PMC
Author	Maertens, Yvonne Humberg, Verena Erlmeier, Franziska Steffens, Sandra Steinestel, Julie Bögemann, Martin Schrader, Andres Jan Bernemann, Christof
Copyright Year	2017
Abstract	BackgroundAnalysis of circulating tumor cells (CTCs) has progressed in several tumor entities. However, little is known about CTCs in clear cell renal cell carcinoma (ccRCC) patients. Aim of our studies was to build a stable in vitro fundament for isolation of CTCs in ccRCC.MethodsWe compared the analytical performance of different CTC isolation methods with regard to yield and purity: EpCAM based enrichment, leukocyte depletion and size based enrichment. EpCAM and cytokeratin 8 (KRT8) as biomarker for CTCs expression were evaluated in ccRCC cell lines as well as clinical samples.ResultsWhile the EpCAM based approach failed to successfully isolate tumor cells, CD45 based approaches showed intermediate recovery rates. The cell-size based Parsortix system showed highest recovery rates. EpCAM expression was low or absent in most cell lines as well as in clinical samples, whereas KRT8 was detected as a potential biomarker in ccRCC.ConclusionEpCAM based approaches might miss a high number of CTCs due to low or absent expression of EpCAM in ccRCC, as shown in cell lines as well as in patient samples. We identified the cell-sized based, label independent Parsortix system to be the most effective recovery system for ccRCC CTCs.
Journal	Oncotarget
Volume Number	8
PubMed Central reference number	PMC5675666
Issue Number	50
PubMed reference number	29152114
e-ISSN	19492553
DOI	10.18632/oncotarget.21197
Language	English
Publisher	Impact Journals LLC
Publisher Date	2017-09-23
Access Restriction	Open
Rights License	This article is distributed under the terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited. Copyright: © 2017 Maertens et al.
Subject Keyword	clear cell renal cell carcinoma circulating tumor cells biomarker liquid biopsy genitourinary cancer
Content Type	Text
Resource Type	Article
Subject	Oncology

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