NDLI: Neuropilin-1 is a glial cell line-derived neurotrophic factor receptor in glioblastoma.

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Neuropilin-1 is a glial cell line-derived neurotrophic factor receptor in glioblastoma.

Content Provider	Europe PMC
Author	Sun, Shen Lei, Yu Li, Qi Wu, Yue Zhang, Lin Mu, Pei-Pei Ji, Guang-Quan Tang, Chuan-Xi Wang, Yu-Qian Gao, Jian Gao, Jin Li, Li Zhuo, Lang Li, Yun-Qing Gao, Dian-Shuai
Copyright Year	2017
Abstract	The aim of this study was to identify the receptor for glial cell line-derived neurotrophic factor (GDNF) in glioblastoma multiforme (GBM). After GST pull-down assays, membrane proteins purified from C6 rat glioma cells were subjected to liquid chromatography-tandem mass spectrometry (LC-MS/MS). The differentially expressed proteins were annotated using Gene Ontology, and neuropilin-1 (NRP1) was identified as the putative GDNF receptor in glioma. NRP1 was more highly expressed in human GBM brains and C6 rat glioma cells than in normal human brains or primary rat astrocytes. Immunofluorescence staining showed that NRP1 was recruited to the membrane by GDNF, and NRP1 co-immunoprecipitated with GDNF. Using the NRP1 and GDNF protein structures to assess molecular docking in the ZDOCK server and visualization with the PyMOL Molecular Graphics System revealed 8 H-bonds and stable positive and negative electrostatic interactions between NRP1 and GDNF. RNAi knockdown of NRP1 reduced proliferation of C6 glioma cells when stimulated with GDNF. NRP1 was an independent risk factor for both survival and recurrence in GBM patients. High NRP1 mRNA expression correlated with shorter OS and DFS (OS: χ2=4.6720, P=0.0307; DFS: χ2=11.013, P=0.0009). NRP1 is thus a GDNF receptor in glioma cells and a potential therapeutic target.
Journal	Oncotarget
Volume Number	8
PubMed Central reference number	PMC5650320
Issue Number	43
PubMed reference number	29088765
e-ISSN	19492553
DOI	10.18632/oncotarget.18630
Language	English
Publisher	Impact Journals LLC
Publisher Date	2017-06-27
Access Restriction	Open
Rights License	This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Copyright: © 2017 Sun et al.
Subject Keyword	glial cell line-derived neurotrophic factor glioblastoma membrane receptor neuropilin-1 cell proliferation
Content Type	Text
Resource Type	Article
Subject	Oncology

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