NDLI: Mechanism of EGCG promoting apoptosis of MCF-7 cell line in human breast cancer.

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Mechanism of EGCG promoting apoptosis of MCF-7 cell line in human breast cancer.

Content Provider	Europe PMC
Author	Huang, Chao-You Han, Zheng Li, Xi Xie, Hui-Hua Zhu, Shan-Shan
Copyright Year	2017
Abstract	The aim of the present study was to investigate the effects of epigallocatechin-3-gallate (EGCG) on the apoptosis of the human MCF-7 cancer cell line and the underlying mechanism. MCF-7 cells were divided into the control group and EGCG groups. The proliferation of MCF-7 cells in the two groups was determined using MTT and apoptosis was examined using flow cytometry. Western blot analysis and qRT-PCR were used to analyze P53 and Bcl-2 expression levels. The silencing effect of specific siRNA was evaluated using RT-PCR and western blot analysis. P53 and Bcl-2 expression levels were determined using western blot analysis in the si-P53, EGCG and EGCG-combined si-P53 groups. EGCG inhibited the proliferation of MCF-7 cells in a concentration-dependent manner and IC50 was 37.681 mol/l. The apoptotic rates were 1.37 and 5.83% (t=8.9, p=0.0124) in the blank control and treatment groups after treatment with 30 µmol/l EGCG. The RT-qPCR and western blot results demonstrated that the effect of siRNA interference was evident. The expression of P53 in the EGCG-combined si-P53 group was higher than that of the si-P53 group, but lower than the EGCG group. The Bcl-2 expression level in the EGCG-combined si-P53 group was lower than that of the si-P53 group and higher than that of the EGCG group. In conclusion, EGCG suppressed the proliferation of human MCF-7 breast cancer cells and promoted apoptosis. In addition, the underlying mechanism may be related to the P53/Bcl-2 signaling pathway.
ISSN	17921074
Journal	Oncology Letters
Volume Number	14
PubMed Central reference number	PMC5588052
Issue Number	3
PubMed reference number	28927122
e-ISSN	17921082
DOI	10.3892/ol.2017.6641
Language	English
Publisher	D.A. Spandidos
Publisher Date	2017-07-20
Access Restriction	Open
Rights License	This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. Copyright: © Huang et al.
Subject Keyword	EGCG MCF-7 apoptosis P53 Bcl-2
Content Type	Text
Resource Type	Article
Subject	Cancer Research Oncology

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