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IL-17A Inhibits Osteogenic Differentiation of Bone Mesenchymal Stem Cells via Wnt Signaling Pathway.
| Content Provider | Europe PMC |
|---|---|
| Author | Wang, Zhenguo Jia, Ying Du, Fu Chen, Min Dong, Xiuhua Chen, Yan Huang, Wen |
| Copyright Year | 2017 |
| Abstract | BackgroundInterleukin-17A (IL-17A) is not only an important modulator of inflammatory reactions, but also affects bone metabolism, which is involved in osteogenic differentiation of stem cells. However, the role and mechanism of IL-17A in osteogenic differentiation of bone mesenchymal stem cells (BMSCs) are not fully understood. In this study, we investigated the role and mechanism of IL-17A in osteogenic differentiation of BMSCs.Material/MethodsThe osteogenic differentiation of BMSCs was induced by osteoblast-induction medium with IL-17A or without IL-17A. The osteogenic differentiation of BMSCs was confirmed by the alkaline phosphatase and alizarin red staining. The lentiviral plasmid was used to construct the sFRP1-shRNA expression vector. The associated osteogenic differentiation marks (RUNX2, ALP, OPN), Wnt signaling pathway inhibitor (sFRP1), and modulators of Wnt signaling pathway (Wnt3, Wnt6) were detected by qRT-PCR and Western blot method.ResultsThe results showed that the addition of IL-17A inhibited osteogenic differentiation of BMSCs. IL-17A induced up-regulated expression of sFRP1 and down-regulated expression of Wnt3 and Wnt6 in BMSCs. In addition, sFRP1-shRNA abolished the inhibition effect of IL-17A in osteogenic differentiation of BMSCs and induced up-regulated expression of Wnt3 and Wnt6 in the Wnt signaling pathway in BMSCs.ConclusionsOur findings show that IL-17A inhibits osteogenic differentiation of bone mesenchymal stem cells via the Wnt signaling pathway. |
| ISSN | 12341010 |
| Journal | Medical Science Monitor : International Medical Journal of Experimental and Clinical Research |
| Volume Number | 23 |
| PubMed Central reference number | PMC5580517 |
| PubMed reference number | 28837545 |
| e-ISSN | 16433750 |
| DOI | 10.12659/msm.903027 |
| Language | English |
| Publisher | International Scientific Literature, Inc. |
| Publisher Date | 2017-08-24 |
| Access Restriction | Open |
| Rights License | This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) © Med Sci Monit, 2017 |
| Subject Keyword | Cell Differentiation Receptors, Interleukin-17 Stem Cells Wnt Signaling Pathway |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine |
