Loading...
Please wait, while we are loading the content...
Similar Documents
Protein kinase C β inhibits autophagy and sensitizes cervical cancer Hela cells to cisplatin.
| Content Provider | Europe PMC |
|---|---|
| Author | Li, Na Zhang, Wei |
| Copyright Year | 2017 |
| Abstract | Recently, autophagy has been indicated to play an essential role in various biological events, such as the response of cervical cancer cells to chemotherapy. However, the exact signalling mechanism that regulates autophagy during chemotherapy remains unclear. In the present study, we investigated the regulation by cisplatin on protein kinase C β (PKC β), on B-cell lymphoma 2 (Bcl-2) and on apoptosis in cervical cancer Hela cells. And then we examined the regulation by cisplatin on autophagy and the role of autophagy on the chemotherapy in Hela cells. In addition, the regulation of the PKC β on the autophagy was also investigated. Our results indicated that cisplatin promoted PKC β in Hela cells. The PKC β inhibitor reduced the cisplatin-induced apoptosis, whereas increased the cisplatin-induced autophagy in Hela cells. On the other side, the PKC β overexpression aggravated the cisplatin-induced apoptosis, whereas down-regulated the cisplatin-induced autophagy. Taken together, our study firstly recognized the involvement of PKC β in the cytotoxicity of cisplatin via inhibiting autophagy in cervical cancer cells. We propose that PKC β would sensitize cervical cancer cells to chemotherapy via reducing the chemotherapy induced autophagy in cancer cells. |
| Page Count | 12 |
| ISSN | 01448463 |
| Volume Number | 37 |
| PubMed Central reference number | PMC5469325 |
| Issue Number | 2 |
| PubMed reference number | 28246354 |
| Journal | Bioscience Reports [Biosci. Rep] |
| e-ISSN | 15734935 |
| DOI | 10.1042/BSR20160445 |
| Language | English |
| Publisher | Portland Press Ltd. |
| Publisher Date | 2017-03-27 |
| Access Restriction | Open |
| Rights License | This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY). © 2017 The Author(s). |
| Subject Keyword | Autophagy Chemotherapy Cisplatin Protein kinase C β |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Molecular Biology Biochemistry Biophysics |
