Naringin enhances osteogenic differentiation through the activation of ERK signaling in human bone marrow mesenchymal stem cells.

Content Provider	Europe PMC
Author	Wang, Huichao Li, Chunbo Li, Jianming Zhu, Yingjie Jia, Yudong Zhang, Ying Zhang, Xiaodong Li, Wenlong Cui, Lei Li, Wuyin Liu, Youwen
Copyright Year	2017
Abstract	Objective(s):Naringin has been reported to regulate bone metabolism. However, its effect on osteogenesis remains unclear. The aim was to investigate the effect of naringin on osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs) through the activation of the ERK signaling pathway in osteogenic differentiation.Materials and Methods:Annexin V-FITC assay and MTT assay were used to measure the effect of naringin on cytotoxicity and proliferation of hBMSCs, respectively. Alkaline phosphatase activity analysis, Alizarin Red S staining, Western blotting, and real-time PCR assay were used to evaluate both the potential effect of naringin on osteogenic differentiation and the role of ERK signaling pathway in osteogenic differentiation.Results:Our results showed that naringin had no obvious toxicity on hBMSCs, and could significantly promote the proliferation of hBMSCs. Naringin also enhanced the osteogenic differentiation of hBMSCs and increased the protein and mRNA expression levels of osteogenic markers such as Runx-2, OXS, OCN, and Col1 in a dose-dependent manner. In addition, we found that the enhancing effect of naringin on osteogenic differentiation was related to the activation of phosphor-ERK, with an increase in duration of activity from 30 min to 120 min. More importantly, both the enhancing effect of naringin on osteogenic differentiation and the activity effect of naringin on ERK signaling pathway were reversed by U0126 addition.Conclusion:Our findings demonstrated that naringin promoted proliferation and osteogenesis of hBMSCs by activating the ERK signaling pathway and it might be a potential therapeutic agent for treating or preventing osteoporosis.
ISSN	20083866
Journal	Iranian Journal of Basic Medical Sciences
Volume Number	20
PubMed Central reference number	PMC5425923
Issue Number	4
PubMed reference number	28804610
e-ISSN	20083874
DOI	10.22038/IJBMS.2017.8582
Language	English
Publisher	Mashhad University of Medical Sciences
Publisher Date	2017-04-01
Publisher Place	Iran
Access Restriction	Open
Rights License	This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Copyright: © Iranian Journal of Basic Medical Sciences
Subject Keyword	Bone marrow-mesenchymal stem cells ERK signaling pathway Naringin Osteogenic differentiation
Content Type	Text
Resource Type	Article
Subject	Immunology and Microbiology Drug Discovery Clinical Biochemistry Biochemistry Biophysics