MoJMJ1, Encoding a Histone Demethylase Containing JmjC Domain, Is Required for Pathogenic Development of the Rice Blast Fungus, Magnaporthe oryzae.

Content Provider	Europe PMC
Author	Huh, Aram Dubey, Akanksha Kim, Seongbeom Jeon, Junhyun Lee, Yong-Hwan
Copyright Year	2017
Abstract	Histone methylation plays important roles in regulating chromatin dynamics and transcription in eukaryotes. Implication of histone modifications in fungal pathogenesis is, however, beginning to emerge. Here, we report identification and functional analysis of a putative JmjC-domain-containing histone demethylase in Magnaporthe oryzae. Through bioinformatics analysis, we identified seven genes, which encode putative histone demethylases containing JmjC domain. Deletion of one gene, MoJMJ1, belonging to JARID group, resulted in defects in vegetative growth, asexual reproduction, appressorium formation as well as invasive growth in the fungus. Western blot analysis showed that global H3K4me3 level increased in the deletion mutant, compared to wild-type strain, indicating histone demethylase activity of MoJMJ1. Introduction of MoJMJ1 gene into ΔMojmj1 restored defects in pre-penetration developments including appressorium formation, indicating the importance of histone demethylation through MoJMJ1 during infection-specific morphogenesis. However, defects in penetration and invasive growth were not complemented. We discuss such incomplete complementation in detail here. Our work on MoJMJ1 provides insights into H3K4me3-mediated regulation of infection-specific development in the plant pathogenic fungus.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC5378440&blobtype=pdf
ISSN	15982254
Journal	The Plant Pathology Journal [Plant Pathol J]
Volume Number	33
PubMed Central reference number	PMC5378440
Issue Number	2
PubMed reference number	28381966
e-ISSN	20939280
DOI	10.5423/ppj.oa.11.2016.0244
Language	English
Publisher	Korean Society of Plant Pathology
Publisher Date	2017-04-01
Access Restriction	Open
Rights License	This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. © The Korean Society of Plant Pathology
Subject Keyword	histone demethylase Magnaporthe oryzae pathogenicity rice blast
Content Type	Text
Resource Type	Article
Subject	Agronomy and Crop Science