NDLI: Detection and localization of viral infection in the pancreas of patients with type 1 diabetes using short fluorescently-labelled oligonucleotide probes.

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Detection and localization of viral infection in the pancreas of patients with type 1 diabetes using short fluorescently-labelled oligonucleotide probes.

Content Provider	Europe PMC
Author	Busse, Niels Paroni, Federico Richardson, Sarah J. Laiho, Jutta E. Oikarinen, Maarit Frisk, Gun Hyöty, Heikki de Koning, Eelco Morgan, Noel G. Maedler, Kathrin
Copyright Year	2017
Abstract	Enteroviruses, specifically of the Coxsackie B virus family, have been implicated in triggering islet autoimmunity and type 1 diabetes, but their presence in pancreata of patients with diabetes has not been fully confirmed.To detect the presence of very low copies of the virus genome in tissue samples from T1D patients, we designed a panel of fluorescently labeled oligonucleotide probes, each of 17-22 nucleotides in length with a unique sequence to specifically bind to the enteroviral genome of the picornaviridae family.With these probes enteroviral RNA was detected with high sensitivity and specificity in infected cells and tissues, including in FFPE pancreas sections from patients with T1D. Detection was not impeded by variations in sample processing and storage thereby overcoming the potential limitations of fragmented RNA. Co-staining of small RNA probes in parallel with classical immunstaining enabled virus detection in a cell-specific manner and more sensitively than by viral protein.
Journal	Oncotarget
Volume Number	8
PubMed Central reference number	PMC5355040
Issue Number	8
PubMed reference number	28147344
e-ISSN	19492553
DOI	10.18632/oncotarget.14896
Language	English
Publisher	Impact Journals LLC
Publisher Date	2017-02-01
Access Restriction	Open
Rights License	This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Copyright: © 2017 Busse et al.
Subject Keyword	enteroviruses type 1 diabetes pancreas islets oligonucleotide probes Pathology Section
Content Type	Text
Resource Type	Article
Subject	Oncology

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