NDLI: Crystallographic snapshots of active site metal shift in E. coli fructose 1,6-bisphosphate aldolase.

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Crystallographic snapshots of active site metal shift in E. coli fructose 1,6-bisphosphate aldolase.

Content Provider	Europe PMC
Author	Tran, Huyen-Thi Lee, Seon-Hwa Ho, Thien-Hoang Hong, Seung-Hye Huynh, Kim-Hung Ahn, Yeh-Jin Oh, Deok-Kun Kang, Lin-Woo
Copyright Year	2016
Abstract	Fructose 1,6-bisphosphate aldolase (FBA) is important for both glycolysis and gluconeogenesis in life. Class II (zinc dependent) FBA is an attractive target for the development of antibiotics against protozoa, bacteria, and fungi, and is also widely used to produce various high-value stereoisomers in the chemical and pharmaceutical industry. In this study, the crystal structures of class II Escherichia coli FBA (EcFBA) were determined from four different crystals, with resolutions between 1.8 Å and 2.0 Å. Native EcFBA structures showed two separate sites of Zn1 (interior position) and Zn2 (active site surface position) for Zn2+ ion. Citrate and TRIS bound EcFBA structures showed Zn2+ position exclusively at Zn2. Crystallographic snapshots of EcFBA structures with and without ligand binding proposed the rationale of metal shift at the active site, which might be a hidden mechanism to keep the trace metal cofactor Zn2+ within EcFBA without losing it.
ISSN	19766696
Journal	BMB Reports
Volume Number	49
PubMed Central reference number	PMC5346313
Issue Number	12
PubMed reference number	27733232
e-ISSN	1976670X
DOI	10.5483/BMBRep.2016.49.12.132
Language	English
Publisher	Korean Society for Biochemistry and Molecular Biology
Publisher Date	2016-12-01
Access Restriction	Open
Rights License	This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Copyright © 2016 by the The Korean Society for Biochemistry and Molecular Biology
Subject Keyword	Class II fructose 1,6-bisphosphate aldolase Inorganic cofactor Metal ion X-ray crystallography
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Biochemistry

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