Green tea polyphenols induce cell death in breast cancer MCF-7 cells through induction of cell cycle arrest and mitochondrial-mediated apoptosis.

Content Provider	Europe PMC
Author	Liu, Shu-min Ou, Shi-yi Huang, Hui-hua
Copyright Year	2017
Description	In order to study the molecular mechanisms of green tea polyphenols (GTPs) in treatment or prevention of breast cancer, the cytotoxic effects of GTPs on five human cell lines (MCF-7, A549, Hela, PC3, and HepG2 cells) were determined and the antitumor mechanisms of GTPs in MCF-7 cells were analyzed. The results showed that GTPs exhibited a broad spectrum of inhibition against the detected cancer cell lines, particularly the MCF-7 cells. Studies on the mechanisms revealed that the main modes of cell death induced by GTPs were cell cycle arrest and mitochondrial-mediated apoptosis. Flow cytometric analysis showed that GTPs mediated cell cycle arrest at both G1/M and G2/M transitions. GTP dose dependently led to apoptosis of MCF-7 cells via the mitochondrial pathways, as evidenced by induction of chromatin condensation, reduction of mitochondrial membrane potential (ΔΨ m), improvement in the generation of reactive oxygen species (ROS), induction of DNA fragmentation, and activations of caspase-3 and caspase-9 in the present paper.
Abstract	In order to study the molecular mechanisms of green tea polyphenols (GTPs) in treatment or prevention of breast cancer, the cytotoxic effects of GTPs on five human cell lines (MCF-7, A549, Hela, PC3, and HepG2 cells) were determined and the antitumor mechanisms of GTPs in MCF-7 cells were analyzed. The results showed that GTPs exhibited a broad spectrum of inhibition against the detected cancer cell lines, particularly the MCF-7 cells. Studies on the mechanisms revealed that the main modes of cell death induced by GTPs were cell cycle arrest and mitochondrial-mediated apoptosis. Flow cytometric analysis showed that GTPs mediated cell cycle arrest at both G1/M and G2/M transitions. GTP dose dependently led to apoptosis of MCF-7 cells via the mitochondrial pathways, as evidenced by induction of chromatin condensation, reduction of mitochondrial membrane potential (ΔΨm), improvement in the generation of reactive oxygen species (ROS), induction of DNA fragmentation, and activations of caspase-3 and caspase-9 in the present paper.
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ISSN	16731581
Volume Number	18
PubMed Central reference number	PMC5296226
Issue Number	2
PubMed reference number	28124838
Journal	Journal of Zhejiang University. Science. B [J Zhejiang Univ Sci B]
e-ISSN	18621783
DOI	10.1631/jzus.b1600022
Language	English
Publisher	Zhejiang University Press
Publisher Date	2017-02-01
Publisher Place	Hangzhou
Access Restriction	Open
Rights License	Copyright © Zhejiang University and Springer-Verlag Berlin Heidelberg 2017
Subject Keyword	Green tea polyphenol (GTP) Breast cancer MCF-7 cells Mitochondrial-mediated apoptosis Cell death Cell cycle arrest
Content Type	Text
Resource Type	Article
Subject	Veterinary Medicine Biochemistry, Genetics and Molecular Biology Pharmacology, Toxicology and Pharmaceutics