Evaluation of Estrogenic Activity of Extract from the Herbal Mixture Cynanchum wilfordii Hemsley, Phlomis umbrosa Turczaninow, and Angelica gigas Nakai.

Content Provider	Europe PMC
Author	Kim, Se Jong Jin, Sun Woo Lee, Gi-Ho Kim, Yong An Jeong, Hye Gwang
Copyright Year	2017
Abstract	Hormone replacement therapy (HRT) consists of highly effective prescription medications for treating menopausal symptoms; however, these agents have exhibited side effects including the risk of estrogen-induced carcinogenesis. Therefore, interest in phytotherapy-based materials as a natural source of alternatives to estrogen therapy has increased. However, some of these herbal medicines have been reported to increase the risk of estrogen-induced cancer. Herbal formulations composed of a combination of Cynanchum wilfordii Hemsley (CW), Phlomis umbrosa Turczaninow (PU), and Angelica gigas Nakai (AG) extracts (CPAE) have been used for treating menopausal symptoms. Therefore, in this study, we aimed to examine the safety of CPAE by determining its potential adverse estrogenic activity using the Organization for Economic Cooperation and Development (OECD) test guideline 455 (TG455) in a stably transfected transcriptionally activated human estrogen receptor α (hERα)-HeLa9903 cell model. We found that CPAE did not how any estrogenic activity or stimulate promoters containing estrogen response elements in MCF-7 cells. In addition, CPAE showed no significant selective activity against hERα and hERβ, non-selective activity against the ER, or effects on ER target gene expression. Furthermore, CPAE did not significantly induce MCF-7 cell proliferation and uterine weight increase in ovariectomized rats. These results demonstrate that CPAE can be used as beneficial herbal drug for prevention and therapeutic intervention of estrogen carcinogenesis in menopausal women.
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ISSN	19768257
Journal	Toxicological Research [Toxicol Res]
Volume Number	33
PubMed Central reference number	PMC5266372
Issue Number	1
PubMed reference number	28133516
e-ISSN	22342753
DOI	10.5487/tr.2017.33.1.071
Language	English
Publisher	Korean Society of Toxicology
Publisher Date	2017-01-15
Access Restriction	Open
Rights License	This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Copyright © 2017 The Korean Society Of Toxicology
Subject Keyword	Cynanchum wilfordii Hemsley Phlomis umbrosa Turczaninow Angelica gigas Nakai Estrogenic activity Estrogen receptor
Content Type	Text
Resource Type	Article
Subject	Health, Toxicology and Mutagenesis Toxicology