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Promoter Polymorphism of Toll-Like Receptor 4 is Associated with a Decreased Risk of Coronary Artery Disease: A Case-Control Study in the Chinese Han Population.
| Content Provider | Europe PMC |
|---|---|
| Author | Sun, Dandan Sun, Liping Xu, Qian Wang, Honghu Yang, Jun Yuan, Yuan |
| Copyright Year | 2017 |
| Abstract | BackgroundCoronary artery disease (CAD) is considered a chronic inflammatory disease of the blood vessels. Toll-like receptor 4 (TLR4) is a transmembrane receptor involved in inflammatory reactions. The aim of this study was to determine the association between polymorphisms in the promoter region and 3′-untranslated region (3′-UTR) of TLR4, and the associated CAD risk.Material/MethodsThis study enrolled 424 participants with CAD and 424 controls without CAD. The polymorphisms in the promoter region and 3′-UTR of TLR4 were identified from the HapMap database, including rs10116253, rs10983755, and rs11536889. Genomic DNA was extracted from peripheral blood. Polymerase chain reaction-restriction fragment length polymorphism was performed to identify genotype polymorphisms. Relative luciferase activity was measured using the dual-luciferase reporter assay system.ResultsTLR4 rs10116253 in the promoter region was associated with CAD risk. The variant (CC+TC) genotypes of rs10116253 were associated with a decreased CAD risk (OR 95% CI 0.73 (0.54–0.98), p=0.034). In the stratification analyses, the variant (CC+TC) genotypes of rs10116253 were observed to have a relationship with decreased CAD risk in the male subgroup (OR: 95% CI 0.68 (0.48–0.98), p=0.041). Moreover, the variant CC and (CC+TC) genotypes of rs10116253 were correlated with a decreased CAD risk in participants younger than 60-year-old (TC: OR (95% CI 0.62 (0.39–0.98), p=0.042; TC+CC: OR 95% CI 0.63 (0.41–0.98), p=0.039). Regarding rs10116253, the luciferase activity of the mutant C allele construct was lower than that of the wild T allele construct (5.215±0.009 vs. 5.304±0.041; p=0.087).ConclusionsThe results provided evidence of an association between the TLR4 rs10116253 in the promoter region and a reduced risk of CAD. |
| ISSN | 12341010 |
| Journal | Medical Science Monitor : International Medical Journal of Experimental and Clinical Research |
| Volume Number | 23 |
| PubMed Central reference number | PMC5266207 |
| PubMed reference number | 28092654 |
| e-ISSN | 16433750 |
| DOI | 10.12659/msm.899587 |
| Language | English |
| Publisher | International Scientific Literature, Inc. |
| Publisher Date | 2017-01-16 |
| Access Restriction | Open |
| Rights License | This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) © Med Sci Monit, 2017 |
| Subject Keyword | Coronary Artery Disease Polymorphism, Single Nucleotide Promoter Regions, Genetic Toll-Like Receptor 4 |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine |
