NDLI: Measurement of DNA Length Changes Upon CpG Hypermethylation by Microfluidic Molecular Stretching.

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Measurement of DNA Length Changes Upon CpG Hypermethylation by Microfluidic Molecular Stretching.

Content Provider	Europe PMC
Author	Onoshima, Daisuke Kawakita, Naoko Takeshita, Daiki Niioka, Hirohiko Yukawa, Hiroshi Miyake, Jun Baba, Yoshinobu
Copyright Year	2016
Abstract	Abnormal DNA methylation in CpG-rich promoters is recognized as a distinct molecular feature of precursor lesions to cancer. Such unintended methylation can occur during in vitro differentiation of stem cells. It takes place in a subset of genes during the differentiation or expansion of stem cell derivatives under general culture conditions, which may need to be monitored in future cell transplantation studies. Here we demonstrate a microfluidic device for investigating morphological length changes in DNA methylation. Arrayed polymer chains of single DNA molecules were fluorescently observed by parallel trapping and stretching in the microfluidic channel. This observational study revealed that the shortened DNA length is due to the increased rigidity of the methylated DNA molecule. The trapping rate of the device for DNA molecules was substantially unaffected by changes in the CpG methylation.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC5225679&blobtype=pdf
Page Count	6
Volume Number	9
PubMed Central reference number	PMC5225679
Issue Number	1-2
PubMed reference number	28293464
Journal	Cell Medicine [Cell Med]
e-ISSN	21551790
DOI	10.3727/215517916x693087
Language	English
Publisher	Cognizant Communication Corporation
Publisher Date	2016-09-14
Publisher Place	Elmsford, NY
Access Restriction	Open
Rights License	Copyright © 2016 Cognizant, LLC.
Subject Keyword	DNA methylation Cytosine-guanine dinucleotides (CpG) Microfluidic device Single-molecule detection
Content Type	Text
Resource Type	Article
Subject	Medicine

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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