CRISPR-Cas9: a promising tool for gene editing on induced pluripotent stem cells.

Content Provider	Europe PMC
Author	Kim, Eun Ji Kang, Ki Ho Ju, Ji Hyeon
Copyright Year	2017
Abstract	Recent advances in genome editing with programmable nucleases have opened up new avenues for multiple applications, from basic research to clinical therapy. The ease of use of the technology—and particularly clustered regularly interspaced short palindromic repeats (CRISPR)—will allow us to improve our understanding of genomic variation in disease processes via cellular and animal models. Here, we highlight the progress made in correcting gene mutations in monogenic hereditary disorders and discuss various CRISPR-associated applications, such as cancer research, synthetic biology, and gene therapy using induced pluripotent stem cells. The challenges, ethical issues, and future prospects of CRISPR-based systems for human research are also discussed.
ISSN	12263303
Volume Number	32
PubMed Central reference number	PMC5214730
Issue Number	1
PubMed reference number	28049282
Journal	The Korean Journal of Internal Medicine [Korean J. Intern. Med]
e-ISSN	20056648
DOI	10.3904/kjim.2016.198
Language	English
Publisher	The Korean Association of Internal Medicine
Publisher Date	2017-01-01
Access Restriction	Open
Rights License	This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Copyright © 2017 The Korean Association of Internal Medicine
Subject Keyword	Clustered regularly interspaced short palindromic repeats-Cas9 Clustered regularly interspaced short palindromic repeats Gene editing Induced pluripotent stem cells Genetic therapy
Content Type	Text
Resource Type	Article
Subject	Internal Medicine