Synthetic Fluorophores for Visualizing Biomolecules in Living Systems.

Content Provider	Europe PMC
Author	Martynov, V. I. Pakhomov, A. A. Popova, N. V. Deyev, I. E. Petrenko, A. G.
Copyright Year	2016
Abstract	The last decade has witnessed significant advance in the imaging of livingsystems using fluorescent markers. This progress has been primarily associatedwith the discovery of different spectral variants of fluorescent proteins.However, the fluorescent protein technology has its own limitations and, insome cases, the use of low-molecular-weight fluorophores is preferable. In thisreview, we describe the arsenal of synthetic fluorescent tools that arecurrently in researchers’ hands and span virtually the entire spectrum,from the UV to visible and, further, to the near-infrared region. An overviewof recent advances in site-directed introduction of synthetic fluorophores intotarget cellular objects is provided. Application of these fluorescent probes tothe solution of a wide range of biological problems, in particular, to thedetermination of local ion concentrations and pH in living systems, isdiscussed.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC5199205&blobtype=pdf
ISSN	20758251
Journal	Acta Naturae
Volume Number	8
PubMed Central reference number	PMC5199205
Issue Number	4
PubMed reference number	28050265
Language	English
Publisher	A.I. Gordeyev
Publisher Date	2016-10-01
Access Restriction	Open
Rights License	This is an open-access article distributed under the terms of theCreative Commons Attribution License, which permits unrestricted use,distribution, and reproduction in any medium, provided the original work isproperly cited. Copyright ® 2016 Park-media Ltd.
Subject Keyword	fluorophore fluorescence microscopy site-directed reaction measurement of ion concentration measurement of local pH
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biochemistry Molecular Medicine Biotechnology