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Isolation, identification, and characterization of novel nanovesicles.
| Content Provider | Europe PMC |
|---|---|
| Author | Zhang, Huang-Ge Cao, Pengxiao Teng, Yun Hu, Xin Wang, Qilong Yeri, Ashish S. Zhuang, Xiaoying Samykutty, Abhilash Mu, Jingyao Deng, Zhong-Bin Zhang, Lifeng Mobley, James A. Yan, Jun Van Keuren-Jensen, Kendall Miller, Donald |
| Copyright Year | 2016 |
| Abstract | Extracellular microvesicles (EVs) have been recognized for many potential clinical applications including biomarkers for disease diagnosis. In this study, we identified a major population of EVs by simply screening fluid samples with a nanosizer. Unlike other EVs, this extracellular nanovesicle (named HG-NV, HG-NV stands for HomoGenous nanovesicle as well as for Huang-Ge- nanovesicle) can be detected with a nanosizer with minimal in vitro manipulation and are much more homogenous in size (8–12 nm) than other EVs. A simple filtration platform is capable of separating HG-NVs from peripheral blood or cell culture supernatants. In comparison with corresponding exosome profiles, HG-NVs released from both mouse and human breast tumor cells are enriched with RNAs. Tumor derived HG-NVs are more potent in promoting tumor progression than exosomes. In summary, we identified a major subset of EVs as a previously unrecognized nanovesicle. Tumor cell derived HG-NVs promote tumor progression. Molecules predominantly present in breast tumor HG-NVs have been identified and characterized. This discovery may have implications in advancing both microvesicle biology research and clinical management including potential used as a biomarker. |
| Journal | Oncotarget |
| Volume Number | 7 |
| PubMed Central reference number | PMC5173064 |
| Issue Number | 27 |
| PubMed reference number | 27191656 |
| e-ISSN | 19492553 |
| DOI | 10.18632/oncotarget.9325 |
| Language | English |
| Publisher | Impact Journals LLC |
| Publisher Date | 2016-07-01 |
| Access Restriction | Open |
| Rights License | This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Copyright: © 2016 Zhang et al. |
| Subject Keyword | isolation and identification extracellular microvesicles in vivo predominately population HG-NV exosomes |
| Content Type | Text |
| Resource Type | Article |
| Subject | Oncology |
