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Organization of some repetitive DNAs and B chromosomes in the grasshopper Eumastusia koebelei koebelei (Rehn, 1909) (Orthoptera, Acrididae, Leptysminae).
| Content Provider | Europe PMC |
|---|---|
| Author | Anjos, Allison Loreto, Vilma Cabral-de-Mello, Diogo C. |
| Abstract | AbstractB chromosomes occur in approximately 15% of eukaryotes and are usually heterochromatic and rich in repetitive DNAs. Here we describe characteristics of a B chromosome in the grasshopper Eumastusia koebelei koebelei (Rehn, 1909) through classical cytogenetic methods and mapping of some repetitive DNAs, including multigene families, telomeric repeats and a DNA fraction enriched with repetitive DNAs obtained from DOP-PCR. Eumastusia koebelei koebelei presented 2n=23, X0 and, in one individual, two copies of the same variant of a B chromosome were noticed, which are associated during meiosis. The C-positive blocks were located in the pericentromeric regions of the standard complement and along the entire length of the B chromosomes. Some G+C-rich heterochromatic blocks were noticed, including conspicuous blocks in the B chromosomes. The mapping of 18S rDNA and U2 snDNA revealed only autosomal clusters, and the telomeric probe hybridized in terminal regions. Finally, the DOP-PCR probe obtained from an individual without a B chromosome revealed signals in the heterochromatic regions, including the entire length of the B chromosome. The possible intraspecific origin of the B chromosomes, due to the shared pool of repetitive DNAs between the A and B chromosomes and the possible consequences of their association are discussed. |
| Related Links | https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC4977798&blobtype=pdf |
| ISSN | 19930771 |
| Journal | Comparative Cytogenetics [Comp Cytogenet] |
| Volume Number | 10 |
| PubMed Central reference number | PMC4977798 |
| Issue Number | 2 |
| PubMed reference number | 27551344 |
| e-ISSN | 1993078X |
| DOI | 10.3897/compcytogen.v10i2.7609 |
| Language | English |
| Publisher | Pensoft Publishers |
| Publisher Date | 2016-04-06 |
| Access Restriction | Open |
| Rights License | This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Allison Anjos, Vilma Loreto, Diogo C. Cabral-de-Mello |
| Subject Keyword | Cytogenetics DOP-PCR FISH multigene family Orthoptera |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Plant Science Animal Science and Zoology Biotechnology Insect Science |
