Inflammation Combined with Ischemia Produces Myelin Injury and Plaque-Like Aggregates of Myelin, Amyloid-β and AβPP in Adult Rat Brain.

Content Provider	Europe PMC
Author	Zhan, Xinhua Cox, Christopher Ander, Bradley P. Liu, Dazhi Stamova, Boryana Jin, Lee-Way Jickling, Glen C. Sharp, Frank R.
Editor	de la Torre, Jack
Copyright Year	2015
Abstract	Background: Ischemia, white matter injury, and Alzheimer’s disease (AD) pathologies often co-exist in aging brain. How one condition predisposes to, interacts with, or perhaps causes the others remains unclear.Objectives: To better understand the link between ischemia, white matter injury, and AD, adult rats were administered lipopolysaccharide (LPS) to serve as an inflammatory stimulus, and 24 h later subjected to 20-min focal cerebral ischemia (IS) followed by 30-min hypoxia (H).Methods: Myelin and axonal damage, as well as amyloid-β (Aβ) and amyloid-β protein precursor (AβPP) deposition were examined by Western blot and immunocytochemistry following LPS/IS/H. Findings were compared to the 5XFAD mouse AD brain.Results: Myelin/axonal injury was observed bilaterally in cortex following LPS/IS/H, along with an increase in IL-1, granzyme B, and LPS. AβPP deposition was present in ischemic striatum in regions of myelin loss. Aβ1-42 and AβPP were deposited in small foci in ischemic cortex that co-localized with myelin aggregates. In the 5XFAD mouse AD model, cortical amyloid plaques also co-localized with myelin aggregates.Conclusions: LPS/IS/H produce myelin injury and plaque-like aggregates of myelin. AβPP and Aβ co-localize with these myelin aggregates.
ISSN	13872877
Journal	Journal of Alzheimer's Disease
Volume Number	46
PubMed Central reference number	PMC4878315
Issue Number	2
PubMed reference number	25790832
e-ISSN	18758908
DOI	10.3233/JAD-143072
Language	English
Publisher	IOS Press
Publisher Date	2015-01-01
Publisher Place	Amsterdam, The Netherlands
Access Restriction	Open
Rights License	This article is published online with Open Access and distributed under the terms of the Creative Commons Attribution Non-Commercial License. IOS Press and the authors. All rights reserved
Subject Keyword	Alzheimer’s disease amyloid plaques amyloid-β amyloid-β protein precursor hypoxia lipopolysaccharide myelin myelin basic protein
Content Type	Text
Resource Type	Article
Subject	Neuroscience Clinical Psychology Psychiatry and Mental Health Geriatrics and Gerontology