NDLI: Isolation and purification of beta-lactoglobulin from cow milk.

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Isolation and purification of beta-lactoglobulin from cow milk.

Content Provider	Europe PMC
Author	Aich, Ranjit Batabyal, Subhasis Joardar, Siddhartha Narayan
Copyright Year	2015
Abstract	Aim:The present study was undertaken to standardize a convenient method for isolation and purification of β-lactoglobulin (β-lg) from cow milk keeping its antigenicity intact, so that the purified β-lg can be used for detection of cow milk protein intolerance (CMPI).Materials and Methods:Raw milk was collected from Gir breed of cattle reared in Haringhata Farm, West Bengal. Milk was then converted to skimmed milk by removing fat globules and casein protein was removed by acidification to pH 4.6 by adding 3 M HCl. β-lg was isolated by gel filtration chromatography using Sephacryl S-200 from the supernatant whey protein fraction. Further, β-lg was purified by anion-exchange chromatography in diethylaminoethyl-sepharose. Molecular weight of the purified cattle β-lg was determined by 15 percent one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis and was analyzed by gel documentation system using standard molecular weight marker.Results:The molecular weight of the purified cattle β-lg was detected as 17.44 kDa. The isolated β-lg was almost in pure form as the molecular weight of purified β-lg monomer is 18kDa.Conclusion:The study revealed a simple and suitable method for isolation of β-lg from whey protein in pure form which may be used for detection of CMPI.
ISSN	09728988
Journal	Veterinary World
Volume Number	8
PubMed Central reference number	PMC4774723
Issue Number	5
PubMed reference number	27047145
e-ISSN	22310916
DOI	10.14202/vetworld.2015.621-624
Language	English
Publisher	Veterinary World
Publisher Date	2015-05-15
Publisher Place	India
Access Restriction	Open
Rights License	This article is an open access article licensed under the terms of the Creative Commons Attributin License (http://creative commons.org/licenses/by/2.0) which permits unrestricted use, distribution and reproduction in any medium, provided the work is properly cited. Copyright: © The authors.
Subject Keyword	beta-lactoglobulin ion-exchange chromatography milk protein intolerance sodium dodecyl sulfate polyacrylamide gel electrophoresis whey protein
Content Type	Text
Resource Type	Article
Subject	Veterinary

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