Association Between Helicobacter pylori cagA, babA2 Virulence Factors and Gastric Mucosal Interleukin-33 mRNA Expression and Clinical Outcomes in Dyspeptic Patients.

Content Provider	Europe PMC
Author	Shahi, Heshmat Reiisi, Somayeh Bahreini, Rasol Bagheri, Nader Salimzadeh, Loghman Shirzad, Hedayatollah
Abstract	Helicobacter pylori (H. pylori) infection has been reported in more than half of the world human population. It is associated with gastric inflammation and noticeable infiltration of the immune cells to the stomach mucosa by several cytokines secretion. IL-1β, IL-18 have been shown to contribute to H. pylori induced gastritis, but the details of inflammation and association of virulence factors remain unclear. IL-1 cytokine family has a new additional cytokine, Interleukin-33 (IL-33), which is contemplated to have an important role for host defense against microorganisms. H. pylori virulence factors important in gastritis risk are the cag pathogenicity island (cag-PAI) and babA. This study evaluated IL-33 mucosal mRNA expression levels in infected and uninfected patients and its relationship with bacterial virulence factors cagA, babA2 and type of gastritis. Total RNA was extracted from gastric biopsies of 79 H. pylori-infected patients and 51 H. pylori-negative patients. Mucosal IL-33 mRNA expression levels in gastric biopsies were assessed using real-time PCR. Existence of virulence factors were detected by PCR. IL-33 mRNA expression was significantly higher in biopsies of H. pylori-infected patients compared to H. pylori-uninfected patients (P<0.0001). Also there was a direct relationship between virulence factor bab-A2 and enhancement in IL-33 mRNA expression. Furthermore, IL-33 mRNA expression level was significantly lower in chronic gastritis patients compared with patients with active gastritis (P<0.001). IL-33 may play a crucial role in the inflammatory response and induction of the chronic gastritis and severity of inflammatory changes in the gastric mucosa.
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ISSN	22519637
Journal	International Journal of Molecular and Cellular Medicine [Int J Mol Cell Med]
Volume Number	4
PubMed Central reference number	PMC4769600
Issue Number	4
PubMed reference number	27014647
e-ISSN	22519645
Language	English
Publisher	Babol University of Medical Sciences
Publisher Date	2015-01-01
Publisher Place	Babol, Iran
Access Restriction	Open
Rights License	This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Subject Keyword	Helicobacter pylori gastritis interleukin-33 virulence factor
Content Type	Text
Resource Type	Article
Subject	Cell Biology Genetics Molecular Biology Biochemistry Molecular Medicine Biotechnology