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FluoroNanogold: an important probe for correlative microscopy.
| Content Provider | Europe PMC |
|---|---|
| Author | Takizawa, Toshihiro Powell, Richard D. Hainfeld, James F. Robinson, John M. |
| Abstract | Correlative microscopy is a powerful imaging approach that refers to observing the same exact structures within a specimen by two or more imaging modalities. In biological samples, this typically means examining the same sub-cellular feature with different imaging methods. Correlative microscopy is not restricted to the domains of fluorescence microscopy and electron microscopy; however, currently, most correlative microscopy studies combine these two methods, and in this review, we will focus on the use of fluorescence and electron microscopy. Successful correlative fluorescence and electron microscopy requires probes, or reporter systems, from which useful information can be obtained with each of the imaging modalities employed. The bi-functional immunolabeling reagent, FluoroNanogold, is one such probe that provides robust signals in both fluorescence and electron microscopy. It consists of a gold cluster compound that is visualized by electron microscopy and a covalently attached fluorophore that is visualized by fluorescence microscopy. FluoroNanogold has been an extremely useful labeling reagent in correlative microscopy studies. In this report, we present an overview of research using this unique probe. |
| Related Links | https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC4744603&blobtype=pdf |
| ISSN | 18646158 |
| Volume Number | 8 |
| DOI | 10.1007/s12154-015-0145-1 |
| PubMed Central reference number | PMC4744603 |
| Issue Number | 4 |
| PubMed reference number | 26884817 |
| Journal | Journal of Chemical Biology [J Chem Biol] |
| e-ISSN | 18646166 |
| Language | English |
| Publisher | Springer Berlin Heidelberg |
| Publisher Date | 2015-08-25 |
| Publisher Place | Berlin/Heidelberg |
| Access Restriction | Open |
| Rights License | © Springer-Verlag Berlin Heidelberg 2015 |
| Subject Keyword | FluoroNanogold Nanogold Correlative microscopy Ultrathin cryosections Immunocytochemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Biophysics |
