NDLI: Long-range RNA pairings contribute to mutually exclusive splicing.

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Long-range RNA pairings contribute to mutually exclusive splicing.

Content Provider	Europe PMC
Author	Yue, Yuan Yang, Yun Dai, Lanzhi Cao, Guozheng Chen, Ran Hong, Weiling Liu, Baoping Shi, Yang Meng, Yijun Shi, Feng Xiao, Mu Jin, Yongfeng
Copyright Year	2015
Abstract	Mutually exclusive splicing is an important means of increasing the protein repertoire, by which the Down's syndrome cell adhesion molecule (Dscam) gene potentially generates 38,016 different isoforms in Drosophila melanogaster. However, the regulatory mechanisms remain obscure due to the complexity of the Dscam exon cluster. Here, we reveal a molecular model for the regulation of the mutually exclusive splicing of the serpent pre-mRNA based on competition between upstream and downstream RNA pairings. Such dual RNA pairings confer fine tuning of the inclusion of alternative exons. Moreover, we demonstrate that the splicing outcome of alternative exons is mediated in relative pairing strength-correlated mode. Combined comparative genomics analysis and experimental evidence revealed similar bidirectional structural architectures in exon clusters 4 and 9 of the Dscam gene. Our findings provide a novel mechanistic framework for the regulation of mutually exclusive splicing and may offer potentially applicable insights into long-range RNA–RNA interactions in gene regulatory networks.
ISSN	13558382
Journal	RNA
Volume Number	22
PubMed Central reference number	PMC4691838
Issue Number	1
PubMed reference number	26554032
e-ISSN	14699001
DOI	10.1261/rna.053314.115
Language	English
Publisher	Cold Spring Harbor Laboratory Press
Publisher Date	2015-11-09
Access Restriction	Open
Rights License	This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/. © 2015 Yue et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society
Subject Keyword	mutually exclusive splicing bidirectional RNA pairing serpent Dscam regulatory mechanism
Content Type	Text
Resource Type	Article
Subject	Molecular Biology

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