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Total saponins from Aralia taibaiensis protect against myocardial ischemia/reperfusion injury through AMPK pathway.
| Content Provider | Europe PMC |
|---|---|
| Author | YAN, JIAJIA DUAN, JIALIN WU, XIAOXIAO GUO, CHAO YIN, YING ZHU, YANRONG HU, TIANXIN WEI, GUO WEN, AIDONG XI, MIAOMIAO |
| Copyright Year | 2015 |
| Abstract | It was previously shown that total saponins extracted from Aralia taibaiensis (sAT) have potent antioxidant activities for treating diabetes mellitus and attenuate D-galactose-induced aging. Since diabetes mellitus and aging are closely associated with cardiac dysfunction, particularly ischemic heart disease, sAT may have potential protective activity against myocardial ischemia/reperfusion injury (MI/RI). However, the anti-MI/RI effects of sAT have yet to be examined, and the possible molecular mechanisms remain to be determined. The present study was undertaken to investigate the anti-MI/RI activities of sAT and to elucidate the mechanisms underlying these effects in rats using TUNEL and Hoechst 33258 staining. The results confirmed the cardioprotective effects in vivo and elucidated the potential molecular mechanisms of sAT in vitro. Pretreatment with sAT significantly reduced infarct size, decreased the levels of lactate dehydrogenase and creatine kinase in the serum and blocked apoptosis. In addition, sAT inhibited A/R-induced apoptosis by decreasing DNA strand breaks, caspase-3 activity and cytochrome c release in H9c2 cells. Furthermore, sAT markedly increased the phosphorylation of AMP-activated protein kinase (AMPK) and acetyl CoA carboxylase and elevated the Bcl2/Bcl-2-associated X protein ratio. These effects were blocked by compound C. The results suggested that sAT pretreatment exerts protective effects on myocardial cells in vitro and in vivo against MI/RI-induced apoptosis by activating AMPK pathway. |
| ISSN | 11073756 |
| Journal | International Journal of Molecular Medicine |
| Volume Number | 36 |
| PubMed Central reference number | PMC4678162 |
| Issue Number | 6 |
| PubMed reference number | 26498380 |
| e-ISSN | 1791244X |
| DOI | 10.3892/ijmm.2015.2391 |
| Language | English |
| Publisher | D.A. Spandidos |
| Publisher Date | 2015-10-23 |
| Access Restriction | Open |
| Rights License | This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. Copyright: © Yan et al. |
| Subject Keyword | total saponins Aralia taibaiensis cardioprotection myocardial ischemia/reperfusion injury apoptosis AMP-activated protein kinase pathway |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Medicine |
