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Optimization of Extraction Parameters for Enhanced Production of Ovotransferrin from Egg White for Antimicrobial Applications.
| Content Provider | Europe PMC |
|---|---|
| Author | Alshammari, Eyad M. A. Khan, Saif Jawed, Arshad Adnan, Mohd Khan, Mahvish Nabi, Gowher Lohani, Mohtashim Haque, Shafiul |
| Copyright Year | 2015 |
| Abstract | Ovotransferrin is the second most abundant protein (~12-13% of the total egg protein) in egg white after ovalbumin. Ovotransferrin is a potent natural antimicrobial agent as it possesses antibacterial, antifungal, and antiviral properties and is also the major metal binding protein found in egg, which makes it an industrially important protein. Ovotransferrin was extracted from egg white using its metal (iron) binding properties. In the present study, eggs from two different sources were used (fresh local eggs from domestic household source and poultry eggs from shops) to compare the results and Response Surface Methodology was used for the experiment design and data analysis. The following extraction conditions were optimized so as to maximize the yield of ovotransferrin from egg white: ethanol % (v/v) and pH and volume (mL) of 25 mM FeCl3/50 mL of egg white. A maximum yield of ~85 ± 2.5% was obtained near the optimum extraction conditions. The yield was calculated based on the theoretical value (934 mg) of ovotransferrin in 100 mL of 1.5x diluted egg white solution. Our results suggest that efficient downstream processing may reduce the cost of overall production process of this promising enzyme, making it a natural and cost-effective alternative to the existing chemically synthesized antimicrobial agents. |
| ISSN | 23146133 |
| Journal | Biomed Research International |
| Volume Number | 2015 |
| PubMed Central reference number | PMC4657406 |
| PubMed reference number | 26640801 |
| e-ISSN | 23146141 |
| DOI | 10.1155/2015/934512 |
| Language | English |
| Publisher | Hindawi |
| Publisher Date | 2015-11-10 |
| Access Restriction | Open |
| Rights License | This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Copyright © 2015 Eyad M. A. Alshammari et al. |
| Content Type | Text |
| Resource Type | Article |
| Subject | Immunology and Microbiology Medicine Biochemistry, Genetics and Molecular Biology |
