Trinitrotoluene Induces Endoplasmic Reticulum Stress and Apoptosis in HePG2 Cells.

Content Provider	Europe PMC
Author	Song, Li Wang, Yue Wang, Jun Yang, Fan Li, Xiaojun Wu, Yonghui
Copyright Year	2015
Abstract	BackgroundThis study aims to describe trinitrotoluene (TNT)-induced endoplasmic reticulum stress (ERS) and apoptosis in HePG2 cells.Material/MethodsHePG2 cells were cultured in vitro with 0, 6, 12, or 24 μg/ml TNT solution for 12, 24, and 48 h. Western blotting was performed to detect intracellular ERS-related proteins, including glucose-regulated protein (GRP) 78, GRP94, Caspase 4, p-Jun N-terminal kinase (JNK), and C/EBP homologous protein (CHOP). Real-time PCR was used to measure mRNA expression from the respective genes.ResultsThe expressions of ERS-related proteins GRP78 and GRP94 as well as mRNA and protein expression of ERS signaling apoptotic CHOP in the TNT treatment group were significantly increased. In addition, the mRNA and protein expression levels of ERS-induced apoptotic protein Caspase-4 were significantly increased. Flow cytometry revealed that after TNT treatment, the apoptosis rate also significantly increased.ConclusionsTNT could increase the expression levels of GRP78, GRP94, Caspase-4, and CHOP in HePG2 cells; this increase in protein expression might be involved in HePG2 apoptosis through the induction of the ERS pathway.
ISSN	12341010
Journal	Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
Volume Number	21
PubMed Central reference number	PMC4644021
PubMed reference number	26551326
e-ISSN	16433750
DOI	10.12659/msm.894169
Language	English
Publisher	International Scientific Literature, Inc.
Publisher Date	2015-11-09
Access Restriction	Open
Rights License	This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License © Med Sci Monit, 2015
Subject Keyword	Apoptosis Endoplasmic Reticulum Stress Trinitrotoluene
Content Type	Text
Resource Type	Article
Subject	Medicine