NDLI: Inhibition of microRNA-497 ameliorates anoxia/reoxygenation injury in cardiomyocytes by suppressing cell apoptosis and enhancing autophagy.

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Inhibition of microRNA-497 ameliorates anoxia/reoxygenation injury in cardiomyocytes by suppressing cell apoptosis and enhancing autophagy.

Content Provider	Europe PMC
Author	Li, Xixian Zeng, Zhi Li, Qingman Xu, Qiulin Xie, Jiahe Hao, Huixin Luo, Guangjin Liao, Wangjun Bin, Jianping Huang, Xiaobo Liao, Yulin
Copyright Year	2015
Abstract	MiR-497 is predicted to target anti-apoptosis gene Bcl2 and autophagy gene microtubule-associated protein 1 light chain 3 B (LC3B), but the functional consequence of miR-497 in response to anoxia/reoxygenation (AR) or ischemia/reperfusion (IR) remains unknown. This study was designed to investigate the influences of miR-497 on myocardial AR or IR injury. We noted that miR-497 was enriched in cardiac tissues, while its expression was dynamically changed in murine hearts subjected to myocardial infarction and in neonatal rat cardiomyocytes (NRCs) subjected to AR. Forced expression of miR-497 (miR-497 mimic) induced apoptosis in NRCs as determined by Hoechst staining and TUNEL assay. In response to AR, silencing of miR-497 using a miR-497 sponge significantly reduced cell apoptosis and enhanced autophagic flux. Furthermore, the infarct size induced by IR in adenovirus (Ad)-miR-497 sponge infected mice was significantly smaller than in mice receiving Ad-vector or vehicle treatment, while Ad-miR-497 increased infarct size. The expression of Bcl-2 and LC3B-II in NRCs or in murine heart was significantly decreased by miR-497 mimic and enhanced by miR-497 sponge. These findings demonstrate that inhibition of miR-497 holds promise for limiting myocardial IR injury.
Journal	Oncotarget
Volume Number	6
PubMed Central reference number	PMC4643066
Issue Number	22
PubMed reference number	26299920
e-ISSN	19492553
DOI	10.18632/oncotarget.4774
Language	English
Publisher	Impact Journals LLC
Publisher Date	2015-08-01
Access Restriction	Open
Rights License	This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Copyright: © 2015 Li et al.
Subject Keyword	Pathology Section microRNA cardiomyocytes ischemia reperfusion apoptosis autophagy
Content Type	Text
Resource Type	Article
Subject	Oncology

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