NDLI: The external PASTA domain of the essential serine/threonine protein kinase PknB regulates mycobacterial growth.

Please wait, while we are loading the content...

The external PASTA domain of the essential serine/threonine protein kinase PknB regulates mycobacterial growth.

Content Provider	Europe PMC
Author	Turapov, Obolbek Loraine, Jessica Jenkins, Christopher H. Barthe, Philippe McFeely, Daniel Forti, Francesca Ghisotti, Daniela Hesek, Dusan Lee, Mijoon Bottrill, Andrew R. Vollmer, Waldemar Mobashery, Shahriar Cohen-Gonsaud, Martin Mukamolova, Galina V.
Copyright Year	2015
Abstract	PknB is an essential serine/threonine protein kinase required for mycobacterial cell division and cell-wall biosynthesis. Here we demonstrate that overexpression of the external PknB_PASTA domain in mycobacteria results in delayed regrowth, accumulation of elongated bacteria and increased sensitivity to β-lactam antibiotics. These changes are accompanied by altered production of certain enzymes involved in cell-wall biosynthesis as revealed by proteomics studies. The growth inhibition caused by overexpression of the PknB_PASTA domain is completely abolished by enhanced concentration of magnesium ions, but not muropeptides. Finally, we show that the addition of recombinant PASTA domain could prevent regrowth of Mycobacterium tuberculosis, and therefore offers an alternative opportunity to control replication of this pathogen. These results suggest that the PknB_PASTA domain is involved in regulation of peptidoglycan biosynthesis and maintenance of cell-wall architecture.
Journal	Open Biology
Volume Number	5
PubMed Central reference number	PMC4632501
Issue Number	7
PubMed reference number	26136255
e-ISSN	20462441
DOI	10.1098/rsob.150025
Language	English
Publisher	The Royal Society
Publisher Date	2015-07-01
Access Restriction	Open
Rights License	© 2015 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.
Subject Keyword	Mycobacterium tuberculosis serine/threonine protein kinase magnesium muropeptides PknB PASTA domain
Content Type	Text
Resource Type	Article
Subject	Immunology Biochemistry, Genetics and Molecular Biology Neuroscience

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in