NDLI: Protective Effect of Intravenous High Molecular Weight Polyethylene Glycol on Fatty Liver Preservation.

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Protective Effect of Intravenous High Molecular Weight Polyethylene Glycol on Fatty Liver Preservation.

Content Provider	Europe PMC
Author	Bejaoui, Mohamed Pantazi, Eirini Folch-Puy, Emma Panisello, Arnau Calvo, María Pasut, Gianfranco Rimola, Antoni Navasa, Miquel Adam, René Roselló-Catafau, Joan
Copyright Year	2015
Abstract	Ischemia reperfusion injury (IRI) leads to significant tissue damage in liver surgery. Polyethylene glycols (PEGs) are water soluble nontoxic polymers that have proved their effectiveness against IRI. The objective of our study was to investigate the potential protective effects of intravenous administration of a high molecular weight PEG of 35 kDa (PEG 35) in steatotic livers subjected to cold ischemia reperfusion. In this study, we used isolated perfused rat liver model to assess the effects of PEG 35 intravenous administration after prolonged cold ischemia (24 h, 4°C) and after reperfusion (2 h, 37°C). Liver injury was measured by transaminases levels and mitochondrial damage was determined by confocal microscopy assessing mitochondrial polarization (after cold storage) and by measuring glutamate dehydrogenase activity (after reperfusion). Also, cell signaling pathways involved in the physiopathology of IRI were assessed by western blot technique. Our results show that intravenous administration of PEG 35 at 10 mg/kg ameliorated liver injury and protected the mitochondria. Moreover, PEG 35 administration induced a significant phosphorylation of prosurvival protein kinase B (Akt) and activation of cytoprotective factors e-NOS and AMPK. In conclusion, intravenous PEG 35 efficiently protects steatotic livers exposed to cold IRI.
ISSN	23146133
Journal	Biomed Research International
Volume Number	2015
PubMed Central reference number	PMC4620277
PubMed reference number	26543868
e-ISSN	23146141
DOI	10.1155/2015/794287
Language	English
Publisher	Hindawi
Publisher Date	2015-10-12
Access Restriction	Open
Rights License	This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Copyright © 2015 Mohamed Bejaoui et al.
Content Type	Text
Resource Type	Article
Subject	Immunology and Microbiology Medicine Biochemistry, Genetics and Molecular Biology

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