The Same Synaptic Vesicles Originate Synchronous and Asynchronous Transmitter Release.

Content Provider	Europe PMC
Author	Grigoryev, P. N. Zefirov, A. L.
Copyright Year	2015
Abstract	Transmitter release and synaptic vesicle exo- and endocytosis duringhigh-frequency stimulation (20 pulses/s) in the extracellular presence ofdifferent bivalent cations (Ca2+, Sr2+ or Ba2+) were studied in frogcutaneous pectoris nerve-muscle preparations. It was shown inelectrophysiological experiments that almost only synchronous transmitterrelease was registered in a Ca2+-containing solution; a highintensity of both synchronous and asynchronous transmitter release wasregistered in a Sr2+-containing solution, and asynchronous transmitter releasealmost only was observed in a Ba2+-containing solution. It was shown inexperiments with a FM 1-43 fluorescent dye that the synaptic vesicles thatundergo exocytosis-endocytosis during synchronous transmitter release(Ca-solutions) are able to participate in asynchronous exocytosis inBa-solutions. The vesicles that had participated in the asynchronoustransmitter release (Ba-solutions) could subsequently participate in asynchronous release (Ca-solutions). It was shown in experiments with isolatedstaining of recycling and reserve synaptic vesicle pools that both types ofevoked transmitter release originate from the same synaptic vesicle pool. ;
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ISSN	20758251
Journal	Acta Naturae
Volume Number	7
PubMed Central reference number	PMC4610168
Issue Number	3
PubMed reference number	26483963
Language	English
Publisher	A.I. Gordeyev
Publisher Date	2015-07-01
Access Restriction	Open
Rights License	This is an open-access article distributed under the terms of theCreative Commons Attribution License, which permits unrestricted use,distribution, and reproduction in any medium, provided the original work isproperly cited. Copyright ® 2015 Park-media Ltd.
Subject Keyword	motor nerve ending evoked synchronous and asynchronous transmitter release Ca2+, Sr2+, Ba2+ ions synaptic vesicle exocytosis and endocytosis synaptic vesicle pools
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biochemistry Molecular Medicine Biotechnology