NDLI: In Vitro Evaluation of Scaffolds for the Delivery of Mesenchymal Stem Cells to Wounds.

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In Vitro Evaluation of Scaffolds for the Delivery of Mesenchymal Stem Cells to Wounds.

Content Provider	Europe PMC
Author	Wahl, Elizabeth A. Fierro, Fernando A. Peavy, Thomas R. Hopfner, Ursula Dye, Julian F. Machens, Hans-Günther Egaña, José T. Schenck, Thilo L.
Copyright Year	2015
Abstract	Mesenchymal stem cells (MSCs) have been shown to improve tissue regeneration in several preclinical and clinical trials. These cells have been used in combination with three-dimensional scaffolds as a promising approach in the field of regenerative medicine. We compare the behavior of human adipose-derived MSCs (AdMSCs) on four different biomaterials that are awaiting or have already received FDA approval to determine a suitable regenerative scaffold for delivering these cells to dermal wounds and increasing healing potential. AdMSCs were isolated, characterized, and seeded onto scaffolds based on chitosan, fibrin, bovine collagen, and decellularized porcine dermis. In vitro results demonstrated that the scaffolds strongly influence key parameters, such as seeding efficiency, cellular distribution, attachment, survival, metabolic activity, and paracrine release. Chick chorioallantoic membrane assays revealed that the scaffold composition similarly influences the angiogenic potential of AdMSCs in vivo. The wound healing potential of scaffolds increases by means of a synergistic relationship between AdMSCs and biomaterial resulting in the release of proangiogenic and cytokine factors, which is currently lacking when a scaffold alone is utilized. Furthermore, the methods used herein can be utilized to test other scaffold materials to increase their wound healing potential with AdMSCs.
ISSN	23146133
Journal	Biomed Research International
Volume Number	2015
PubMed Central reference number	PMC4609332
PubMed reference number	26504774
e-ISSN	23146141
DOI	10.1155/2015/108571
Language	English
Publisher	Hindawi
Publisher Date	2015-10-04
Access Restriction	Open
Rights License	This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Copyright © 2015 Elizabeth A. Wahl et al.
Content Type	Text
Resource Type	Article
Subject	Immunology and Microbiology Medicine Biochemistry, Genetics and Molecular Biology

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