Histopathological evaluation of the ocular-irritation potential of shampoos, make-up removers and cleansing foams in the bovine corneal opacity and permeability assay.

Content Provider	Europe PMC
Author	Furukawa, Masatoshi Sakakibara, Takashi Itoh, Kouta Kawamura, Kohtaro Sasaki, Satoshi Matsuura, Masao
Copyright Year	2015
Abstract	The bovine corneal opacity and permeability (BCOP) assay is an alternative method to the in vivo Draize eye test in rabbits for evaluating eye irritation in vitro. Here, we compared the numerical results of the BCOP assay with the corresponding histopathology for three different corneas for each test substance, including commercially available shampoos, make-up removers and cleansing foams that contained surfactants and other ingredients. The histopathological score was defined based on the severity of lesions in the corneal epithelium. The histopathological findings and scores of the three sections for each test substance were comparable. The in vitro irritancy score (IVIS) generally corresponds to the corneal irritant potential of the test substances assigned on the basis of the histopathological findings in this study. In the present study, we characterized the histopathology of the corneal epithelium and stroma and especially showed that the corneal epithelial injury caused by test substances might be important in assessment of test substances that are mild eye irritants (category 2B) as classified by the United Nations (UN) Globally Harmonized System of Classification and Labelling of Chemicals (GHS), as corneal lesions suggestive of classification into category 2B were localized on the border between the corneal epithelium and stroma, which contained cell elements related to assessment of prognosis of an in vivo eye injury. Histopathological assessment might be useful in predicting in vivo ocular irritation, particularly for test substances with an IVIS >3.1 but ≤25 that are classified as mild irritants (category 2B) according to the UN GHS.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC4604136&blobtype=pdf
ISSN	09149198
Journal	Journal of Toxicologic Pathology [J Toxicol Pathol]
Volume Number	28
DOI	10.1293/tox.2015-0022
PubMed Central reference number	PMC4604136
Issue Number	4
PubMed reference number	26538816
e-ISSN	1881915X
Language	English
Publisher	Japanese Society of Toxicologic Pathology
Publisher Date	2015-07-31
Access Restriction	Open
Rights License	This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. ©2015 The Japanese Society of Toxicologic Pathology
Subject Keyword	bovine corneal opacity and permeability (BCOP) assay IVIS cosmetic products eye irritation histopathologicalscoring corneal epithelium
Content Type	Text
Resource Type	Article
Subject	Toxicology Pathology and Forensic Medicine