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Imaging stress.
| Content Provider | Europe PMC |
|---|---|
| Author | Brielle, Shlomi Gura, Rotem Kaganovich, Daniel |
| Abstract | Recent innovations in cell biology and imaging approaches are changing the way we study cellular stress, protein misfolding, and aggregation. Studies have begun to show that stress responses are even more variegated and dynamic than previously thought, encompassing nano-scale reorganization of cytosolic machinery that occurs almost instantaneously, much faster than transcriptional responses. Moreover, protein and mRNA quality control is often organized into highly dynamic macromolecular assemblies, or dynamic droplets, which could easily be mistaken for dysfunctional “aggregates,” but which are, in fact, regulated functional compartments. The nano-scale architecture of stress-response ranges from diffraction-limited structures like stress granules, P-bodies, and stress foci to slightly larger quality control inclusions like juxta nuclear quality control compartment (JUNQ) and insoluble protein deposit compartment (IPOD), as well as others. Examining the biochemical and physical properties of these dynamic structures necessitates live cell imaging at high spatial and temporal resolution, and techniques to make quantitative measurements with respect to movement, localization, and mobility. Hence, it is important to note some of the most recent observations, while casting an eye towards new imaging approaches that offer the possibility of collecting entirely new kinds of data from living cells. Electronic supplementary material The online version of this article (doi:10.1007/s12192-015-0615-y) contains supplementary material, which is available to authorized users. |
| Related Links | https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC4595435&blobtype=pdf |
| ISSN | 13558145 |
| Volume Number | 20 |
| DOI | 10.1007/s12192-015-0615-y |
| PubMed Central reference number | PMC4595435 |
| Issue Number | 6 |
| PubMed reference number | 26139131 |
| Journal | Cell Stress & Chaperones |
| e-ISSN | 14661268 |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2015-07-04 |
| Publisher Place | Dordrecht |
| Access Restriction | Open |
| Rights License | © Cell Stress Society International 2015 |
| Subject Keyword | Chaperone Misfolded protein Aggregation FRAP FLIP PhoC Inclusion JUNQ IPOD Stress foci Stress granules P-bodies Dynamic droplets |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry |
