Green synthesis and characterization of Carica papaya leaf extract coated silver nanoparticles through X-ray diffraction, electron microscopy and evaluation of bactericidal properties.

Content Provider	Europe PMC
Author	Banala, Rajkiran Reddy Nagati, Veera Babu Karnati, Pratap Reddy
Copyright Year	2015
Abstract	The evolution of nanotechnology and the production of nanomedicine from various sources had proven to be of intense value in the field of biomedicine. The smaller size of nanoparticles is gaining importance in research for the treatment of various diseases. Moreover the production of nanoparticles is eco-friendly and cost effective. In the present study silver nanoparticles were synthesized from Carica papaya leaf extract (CPL) and characterized for their size and shape using scanning electron microscopy and transmission electron microscopy, respectively. Fourier transform infrared spectroscopy (FTIR), Energy dispersive X-ray spectroscopy (EDS/EDX) and X-ray diffraction spectroscopy (XRD) were conducted to determine the concentration of metal ions, the shape of molecules. The bactericidal activity was evaluated using Luria Bertani broth cultures and the minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) were estimated using turbidimetry. The data analysis showed size of 50–250 nm spherical shaped nanoparticles. The turbidimetry analysis showed MIC and MBC was >25 μg/mL against both Gram positive and Gram negative bacteria in Luria Bertani broth cultures. In summary the synthesized silver nanoparticles from CPL showed acceptable size and shape of nanoparticles and effective bactericidal activity.
ISSN	1319562X
Volume Number	22
PubMed Central reference number	PMC4537873
Issue Number	5
PubMed reference number	26288570
Journal	Saudi Journal of Biological Sciences [Saudi J Biol Sci]
e-ISSN	22137106
DOI	10.1016/j.sjbs.2015.01.007
Language	English
Publisher	Elsevier
Publisher Date	2015-02-12
Access Restriction	Open
Rights License	This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). © 2015 The Authors
Subject Keyword	Silver nanoparticles Carica papaya leaf extract Electron microscopy Fourier transform infrared spectroscopy (FTIR) Energy dispersive X-ray spectroscopy (EDS/EDX) X-ray diffraction spectroscopy (XRD) Bactericidal efficiency
Content Type	Text
Resource Type	Article
Subject	Agricultural and Biological Sciences