Evaluating the Effect of Copper Nanoparticles in Inhibiting Pseudomonas aeruginosa and Listeria monocytogenes Biofilm Formation.

Content Provider	Europe PMC
Author	Ghasemian, Ehsan Naghoni, Ali Rahvar, Helya Kialha, Mahsa Tabaraie, Bahman
Copyright Year	2015
Abstract	Background:Biofilm formation is a major virulence factor in different bacteria. Biofilms allow bacteria to resist treatment with antibacterial agents. The biofilm formation on glass and steel surfaces, which are extremely useful surfaces in food industries and medical devices, has always had an important role in the distribution and transmission of infectious diseases.Objectives:In this study, the effect of coating glass and steel surfaces by copper nanoparticles (CuNPs) in inhibiting the biofilm formation by Listeria monocytogenes and Pseudomonas aeruginosa was examined.Materials and Methods:The minimal inhibitory concentrations (MICs) of synthesized CuNPs were measured against L. monocytogenes and P. aeruginosa by using the broth-dilution method. The cell-surface hydrophobicity of the selected bacteria was assessed using the bacterial adhesion to hydrocarbon (BATH) method. Also, the effect of the CuNP-coated surfaces on the biofilm formation of the selected bacteria was calculated via the surface assay.Results:The MICs for the CuNPs according to the broth-dilution method were ≤ 16 mg/L for L. monocytogenes and ≤ 32 mg/L for P. aeruginosa. The hydrophobicity of P. aeruginosa and L. monocytogenes was calculated as 74% and 67%, respectively. The results for the surface assay showed a significant decrease in bacterial attachment and colonization on the CuNP-covered surfaces.Conclusions:Our data demonstrated that the CuNPs inhibited bacterial growth and that the CuNP-coated surfaces decreased the microbial count and the microbial biofilm formation. Such CuNP-coated surfaces can be used in medical devices and food industries, although further studies in order to measure their level of toxicity would be necessary.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC4537522&blobtype=pdf
ISSN	20083645
Journal	Jundishapur Journal of Microbiology [Jundishapur J Microbiol]
Volume Number	8
DOI	10.5812/jjm.8(5)2015.17430
PubMed Central reference number	PMC4537522
Issue Number	5
PubMed reference number	26290685
e-ISSN	20084161
Language	English
Publisher	Kowsar
Publisher Date	2015-05-31
Access Restriction	Open
Rights License	This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited. Copyright © 2015, Ahvaz Jundishapur University of Medical Sciences.
Subject Keyword	Biofilms Nanoparticles Listeria monocytogenes Pseudomonas aeruginosa
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Microbiology Microbiology (medical)