Relationships among 64k autoantibodies, pancreatic beta-cell function, HLA-DR antigens and HLA-DQ genes in patients with insulin-dependent diabetes mellitus in Korea.

Content Provider	Europe PMC
Author	Lee, Hyun Chul Cha, Bong Soo Nam, Moon Suk Song, Young Duk Lim, Sung Kil Kim, Duk Hi Huh, Kap Bum Koh, In Young
Copyright Year	1995
Abstract	ObjectivesAmong autoantibodies detected in patients with insulin-dependent diabetes mellitus(IDDM), antibodies to 64,000Mr islet protein(64k), now recognized as glutamic acid decarboxy lase(GAD), appear to be an even more predictive marker of IDDM than islet cytoplasmic antibody(ICA) or insulin autoantibody(IAA). We examined the relationships among 64k autoantibodies, pancreatic β-cell function, HLA-DR antigens and HLA-DQ genes in patients with IDDM in Korea.MethodsTo identify the 64k autoantibody, the immunoprecipitation method was performed for 35 patients with IDDM and 10 normal controls. In patients with IDDM, serum C-peptide levels were measured and HLA-DR typings and HLA-DQA1 and DQB1 gene typings were performed.Results12 of 35(34%) patients with IDDM were positive for 64k autoantibody in contrast to none of 10(0%) normal controls. There were no differences in residual pancreatic β-cell function between 64k autoantibody positive and negative groups. 64k autoantibody was detected more frequently in patients with recent(duration< 6 months, 10/25[40%]) and young -aged(age< 15 years, 7/18[39%]) onset of IDDM. All of 3(100%) patients with HLA-DR3/DR4 heterotypes were positive in 64k autoantibody, in contrast to 1 of 7(14%) patients without HLA-DR3 nor DR4. The frequencies of HLA-DQA1*0301, HLA-DQB1*0201, DQB1*0302 and DQB1*0303 gene types were higher in patients with 64k autoantibody (12/12[100%]) vs. without 64k autoantibody 18/22[81%], 5/11[45%] vs. without 64k autoantibody 5/22[23%], 5/11[45%] vs. without 64k autoantibody 8/22[36%] and 6/11[55%] vs. without 64k autoantibody 9/22[41%].ConclusionsThere results suggest that 64k autoantibodies have some relationship with HLA-DR, DQA1 and DQB1 genes, but not with residual pancreatic β-cell function in Korean patients with IDDM.
ISSN	12263303
Volume Number	10
PubMed Central reference number	PMC4532034
Issue Number	1
PubMed reference number	7626550
Journal	The Korean Journal of Internal Medicine [Korean J. Intern. Med]
e-ISSN	20056648
DOI	10.3904/kjim.1995.10.1.1
Language	English
Publisher	Korean Association of Internal Medicine
Publisher Date	1995-01-01
Access Restriction	Open
Rights License	This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Copyright © 1995 The Korean Association of Internal Medicine
Subject Keyword	64k autoantibody Insulin-dependent diabetes mellitus Residual pancreatic β-cell function HLA-DR DQA1 DQB1 Korea
Content Type	Text
Resource Type	Article
Subject	Internal Medicine