Soluble extract of soybean fermented with Aspergillus oryzae GB107 inhibits fat accumulation in cultured 3T3-L1 adipocytes.

Content Provider	Europe PMC
Author	So, Kyoung-Ha Suzuki, Yasuki Yonekura, Shinichi Suzuki, Yutaka Lee, Chan Ho Kim, Sung Woo Katoh, Kazuo Roh, Sang-Gun
Copyright Year	2015
Abstract	BACKGROUND/OBJECTIVESThis study was conducted to investigate the effects of fermented soybean (FS) extract on adipocyte differentiation and fat accumulation using cultured 3T3-L1 adipocytes.MATERIALS/METHODS3T3-L1 adipocytes were treated with FS and nonfermented soybean (NFS) extract during differentiation for 10 days in vitro. Oil red O staining was performed and glycerol-3-phosphate dehydrogenase (GPDH) activity was measured for analysis of fat accumulation. Expressions of adipogenic genes were measured.RESULTSSoluble extract of soybean fermented with Aspergillus oryzae GB107 contained higher levels of low-molecular-weight protein than conventional soybean protein did. FS extract (50 µg/ml) inhibited adipocyte differentiation and fat accumulation during differentiation of 3T3-L1 preadipocytes for 10 days in vitro. Significantly lower GPDH activity was observed in differentiated adipocytes treated with the FS extract than those treated with NFS extract. Treatment with FS extract resulted in decreased expression levels of leptin, adiponectin, and adipogenin genes, which are associated with adipogenesis.CONCLUSIONSThis report is the first to demonstrate that the water-soluble extract from FS inhibits fat accumulation and lipid storage in 3T3-L1 adipocytes. Thus, the soybean extract fermented with A. oryzae GB107 could be used to control lipid accumulation in adipocytes.
ISSN	19761457
Journal	Nutrition Research and Practice
Volume Number	9
PubMed Central reference number	PMC4523490
Issue Number	4
PubMed reference number	26244085
e-ISSN	20056168
DOI	10.4162/nrp.2015.9.4.439
Language	English
Publisher	The Korean Nutrition Society and the Korean Society of Community Nutrition
Publisher Date	2015-05-22
Access Restriction	Open
Rights License	This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. ©2015 The Korean Nutrition Society and the Korean Society of Community Nutrition
Subject Keyword	Fermented soybean adipocyte adipogenesis 3T3-L1 Aspergillus oryzae
Content Type	Text
Resource Type	Article
Subject	Nutrition and Dietetics Food Science