Promoting Effects on Proliferation and Chondrogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells by Four "Kidney-Tonifying" Traditional Chinese Herbs.

Content Provider	Europe PMC
Author	Cai, Bin Zhang, Ai-guo Zhang, Xian Ge, Wen-jie Dai, Guo-da Tan, Xiang-ling Roodrajeetsing, Gopaul Cai, Jian-ping
Copyright Year	2015
Abstract	Traditional Chinesemedicine can promote the proliferation of bonemarrow-derived mesenchymal stem cells (BMSCs).We chose four “Kidney-tonifying”Chinese herbal medicines, Radix Astragali, Salvia,Herba Epimedii, and Saussurea Involucrata, toevaluate whether they had positive effects onthe proliferation of BMSCs andTGF-β1-induced chondrogenicdifferentiation of BMSCs. The four Chineseherbal medicines were intragastricallyadministered to Sprague-Dawley rats,respectively, to prepare drug-containing serumsof corresponding Chinese herbs. BMSCs wereisolated, cultured, and exposed to culturesolution containing 1%, 5%, 10%,and 15% (v/v) Radix Astragali-, Salvia-,Herba Epimedii-, and SaussureaInvolucrata-containing serum, respectively.TGF-β1-induced BMSCs were addressed in thesame manner. Collagen type II protein wasassessed by immunofluorescence methods. Toassess whether the drug-containing serums hadpositive effects on the proliferation of BMSCsand TGF-β1-induced BMSCs, MTT method wasassessed. The proliferation of BMSCs wassignificantly enhanced when exposed to culturesolutions containing 1% and 5% RadixAstragali-, 1% and 5% Salvia-, 5%Herba Epimedii-, and 1%, 5%, and10% Saussurea Involucrata-containing serum.The proliferation of TGF-β1-induced BMSCswas significantly enhanced when exposed to1%, 5%, and 15% Radix Astragali-,10% and 15% Salvia-, 5%, and15% Herba Epimedii-, and 1%, 5%,and 10% Saussurea Involucrata-containingserum.
ISSN	23146133
Journal	Biomed Research International
Volume Number	2015
PubMed Central reference number	PMC4475534
PubMed reference number	26137494
e-ISSN	23146141
DOI	10.1155/2015/792161
Language	English
Publisher	Hindawi
Publisher Date	2015-06-07
Access Restriction	Open
Rights License	This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Copyright © 2015 Bin Cai et al.
Content Type	Text
Resource Type	Article
Subject	Immunology and Microbiology Medicine Biochemistry, Genetics and Molecular Biology