NDLI: Anticancer Effect and Apoptosis Induction of Gambogic Acid in Human Leukemia Cell Line K562 In Vitro.

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Anticancer Effect and Apoptosis Induction of Gambogic Acid in Human Leukemia Cell Line K562 In Vitro.

Content Provider	Europe PMC
Author	Chen, Jinhao Zhou, Min Zhang, Qian Xu, Jingyan Ouyang, Jian
Copyright Year	2015
Abstract	BackgroundThe aim of this study was to investigate the anticancer effect and related mechanisms of gambogic acid (GA), a traditional Chinese medicine, on human leukemia cell line K562, together with the effect on bone marrow mononuclear cells (MNCs).Material/MethodsK562 cells and MNCs were treated with various concentrations and treatment times of GA. Inhibitory rate was detected by use of the Cell Counting Kit-8 (CCK-8) assay. Apoptosis was analyzed by morphological detection, Annexin-V/PI doubling staining, and TUNEL assays. The expression changes of pivotal proteins were evaluated by Western blotting.ResultsGA not only suppressed cell proliferation, but also induced apoptosis of K562 cells in a dose-dependent manner. While it did not significantly inhibit cell proliferation of MNCs, it did induce apoptosis in a dose-dependent manner. CCK-8 assay revealed that the proliferation of K562 cells was significantly inhibited when the concentration of GA was more than 0.5 μM. Morphological detection showed the nuclei became denser and more intense orange in K562 cells after GA treatment compared with the untreated group. The expression levels of BCL-2, nuclear factor-κB (NF-κB), c-myc, phosphatidylinositol3-kinase (PI3K), and phosphorylation of serine-threonine kinase (p-AKT) were down-regulated by GA.ConclusionsGA significantly suppressed the proliferation of K562 cells, but has less effect on MNCs. The inhibition of K562 cells proliferation and apoptosis induced by GA might be related to the down-regulation of BCL-2, NF-κB, c-myc, PI3K, and p-AKT.
ISSN	12341010
Journal	Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
Volume Number	21
PubMed Central reference number	PMC4463775
PubMed reference number	26049398
e-ISSN	16433750
DOI	10.12659/msm.893004
Language	English
Publisher	International Scientific Literature, Inc.
Publisher Date	2015-06-02
Access Restriction	Open
Rights License	This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License © Med Sci Monit, 2015
Subject Keyword	Apoptosis Bone Marrow Mononuclear Cells Gambogic Acid Gene Expression Human Leukemia Cell Line K562
Content Type	Text
Resource Type	Article
Subject	Medicine

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