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Jak1/Stat3 is an upstream signaling of NF-κB activation in Helicobacter pylori-induced IL-8 production in gastric epithelial AGS cells.
| Content Provider | Europe PMC |
|---|---|
| Author | Cha, Boram Lim, Joo Weon Kim, Hyeyoung |
| Copyright Year | 2015 |
| Abstract | Helicobacter pylori (H. pylori) induces the activation of nuclear factor-kB (NF-κB) and cytokine expression in gastric epithelial cells. The Janus kinase/signal transducers and activators of transcription (Jak/Stat) cascade is the inflammatory signaling in various cells. The purpose of the present study is to determine whether H. pylori-induced activation of NF-κB and the expression of interleukin-8 (IL-8) are mediated by the activation of Jak1/Stat3 in gastric epithelial (AGS) cells. Thus, gastric epithelial AGS cells were infected with H. pylori in Korean isolates (HP99) at bacterium/cell ratio of 300:1, and the level of IL-8 in the medium was determined by enzyme-linked immonosorbent assay. Phospho-specific and total forms of Jak1/Stat3 and IκBα were assessed by Western blot analysis, and NF-κB activation was determined by electrophoretic mobility shift assay. The results showed that H. pylori induced the activation of Jak1/Stat3 and IL-8 production, which was inhibited by a Jak/Stat3 specific inhibitor AG490 in AGS cells in a dose-dependent manner. H. pylori-induced activation of NF-κB, determined by phosphorylation of IκBα and NF-κB-DNA binding activity, were inhibited by AG490. In conclusion, Jak1/Stat3 activation may mediate the activation of NF-κB and the expression of IL-8 in H. pylori-infected AGS cells. Inhibition of Jak1/Stat3 may be beneficial for the treatment of H. pylori-induced gastric inflammation, since the activation of NF-κB is inhibited and inflammatory cytokine expression is suppressed. |
| ISSN | 05135796 |
| Volume Number | 56 |
| PubMed Central reference number | PMC4397461 |
| Issue Number | 3 |
| PubMed reference number | 25837197 |
| Journal | Yonsei Medical Journal [Yonsei Med. J] |
| e-ISSN | 19762437 |
| DOI | 10.3349/ymj.2015.56.3.862 |
| Language | English |
| Publisher | Yonsei University College of Medicine |
| Publisher Date | 2015-05-01 |
| Access Restriction | Open |
| Rights License | This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. © Copyright: Yonsei University College of Medicine 2015 |
| Subject Keyword | Helicobacter pylori Jak1/Stat3 IL-8 NF-κB gastric epithelial cells |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine |
