NDLI: Two-photon microscopy allows imaging and characterization of cochlear microvasculature in vivo.

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Two-photon microscopy allows imaging and characterization of cochlear microvasculature in vivo.

Content Provider	Europe PMC
Author	Ihler, Friedrich Bertlich, Mattis Weiss, Bernhard Dietzel, Steffen Canis, Martin
Copyright Year	2015
Abstract	Impairment of cochlear blood flow has been discussed as factor in the pathophysiology of various inner ear disorders. However, the microscopic study of cochlear microcirculation is limited due to small scale and anatomical constraints. Here, two-photon fluorescence microscopy is applied to visualize cochlear microvessels. Guinea pigs were injected with Fluorescein isothiocyanate- or Texas red-dextrane as plasma marker. Intravital microscopy was performed in four animals and explanted cochleae from four animals were studied. The vascular architecture of the cochlea was visualized up to a depth of 90.0 ± 22.7 μm. Imaging yielded a mean contrast-to-noise ratio (CNR) of 3.3 ± 1.7. Mean diameter in vivo was 16.5 ± 6.0 μm for arterioles and 8.0 ± 2.4 μm for capillaries. In explanted cochleae, the diameter of radiating arterioles and capillaries was measured with 12.2 ± 1.6 μm and 6.6 ± 1.0 μm, respectively. The difference between capillaries and arterioles was statistically significant in both experimental setups (P < 0.001 and P = 0.022, two-way ANOVA). Measured vessel diameters in vivo and ex vivo were in agreement with published data. We conclude that two-photon fluorescence microscopy allows the investigation of cochlear microvessels and is potentially a valuable tool for inner ear research.
ISSN	23146133
Journal	Biomed Research International
Volume Number	2015
PubMed Central reference number	PMC4390612
PubMed reference number	25883941
e-ISSN	23146141
DOI	10.1155/2015/154272
Language	English
Publisher	Hindawi
Publisher Date	2015-03-30
Access Restriction	Open
Rights License	This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Copyright © 2015 Friedrich Ihler et al.
Content Type	Text
Resource Type	Article
Subject	Immunology and Microbiology Medicine Biochemistry, Genetics and Molecular Biology

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