NDLI: Characterization of bone marrow mononuclear cells on biomaterials for bone tissue engineering in vitro.

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Characterization of bone marrow mononuclear cells on biomaterials for bone tissue engineering in vitro.

Content Provider	Europe PMC
Author	Henrich, Dirk Verboket, René Schaible, Alexander Kontradowitz, Kerstin Oppermann, Elsie Brune, Jan C. Nau, Christoph Meier, Simon Bonig, Halvard Marzi, Ingo Seebach, Caroline
Copyright Year	2015
Abstract	Bone marrow mononuclear cells (BMCs) are suitable for bone tissue engineering. Comparative data regarding the needs of BMC for the adhesion on biomaterials and biocompatibility to various biomaterials are lacking to a large extent. Therefore, we evaluated whether a surface coating would enhance BMC adhesion and analyze the biocompatibility of three different kinds of biomaterials. BMCs were purified from human bone marrow aspirate samples. Beta tricalcium phosphate (β-TCP, without coating or coated with fibronectin or human plasma), demineralized bone matrix (DBM), and bovine cancellous bone (BS) were assessed. Seeding efficacy on β-TCP was 95% regardless of the surface coating. BMC demonstrated a significantly increased initial adhesion on DBM and β-TCP compared to BS. On day 14, metabolic activity was significantly increased in BMC seeded on DBM in comparison to BMC seeded on BS. Likewise increased VEGF-synthesis was observed on day 2 in BMC seeded on DBM when compared to BMC seeded on BS. The seeding efficacy of BMC on uncoated biomaterials is generally high although there are differences between these biomaterials. Beta-TCP and DBM were similar and both superior to BS, suggesting either as suitable materials for spatial restriction of BMC used for regenerative medicine purposes in vivo.
ISSN	23146133
Journal	Biomed Research International
Volume Number	2015
PubMed Central reference number	PMC4352750
PubMed reference number	25802865
e-ISSN	23146141
DOI	10.1155/2015/762407
Language	English
Publisher	Hindawi
Publisher Date	2015-02-23
Access Restriction	Open
Rights License	This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Copyright © 2015 Dirk Henrich et al.
Content Type	Text
Resource Type	Article
Subject	Immunology and Microbiology Medicine Biochemistry, Genetics and Molecular Biology

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