NDLI: Curcumin Suppresses the Production of Pro-inflammatory Cytokine Interleukin-18 in Lipopolysaccharide Stimulated Murine Macrophage-Like Cells.

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Curcumin Suppresses the Production of Pro-inflammatory Cytokine Interleukin-18 in Lipopolysaccharide Stimulated Murine Macrophage-Like Cells.

Content Provider	Europe PMC
Author	Yadav, Renu Jee, Babban Awasthi, Sudhir Kumar
Abstract	Curcumin is a major bioactive compound of turmeric that exerts its anti-inflammatory effects by suppressing the many pro-inflammatory cytokines and chemokines in a number of cell types and pathologic conditions. Interleukin-18 (IL-18) is a novel pro-inflammatory cytokine which plays an important role not only in generating Th1 responses but also in inducing severe inflammatory reactions. As curcumin induced inhibition of IL-18 production in keratinocytes and mice is well known, effect of curcumin on IL-18 release in macrophages remains unknown. Hence, this present study has been designed to evaluate the effect of curcumin on IL-18 production and necrotic cell death in murine macrophages-like cells treated with or without lipopolysaccharide (LPS). The IL-18 secretion in cell culture supernatants was assayed by enzyme-linked immunosorbent assay and cytotoxicity was determined by lactate dehydrogenase release assay. Our results demonstrate that curcumin significantly inhibited the production of pro-inflammatory cytokine IL-18 in E.coli LPS stimulated murine macrophage-like cells RAW264.7 in a concentration-dependent manner. Interestingly, curcumin had no cytotoxic effect on murine macrophage-like cells. Our findings suggest that curcumin may be used as a potential therapeutic agent for the treatment of inflammatory diseases.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC4310836&blobtype=pdf
ISSN	09701915
Volume Number	30
DOI	10.1007/s12291-014-0452-2
PubMed Central reference number	PMC4310836
Issue Number	1
PubMed reference number	25646051
Journal	Indian Journal of Clinical Biochemistry [Indian J Clin Biochem]
e-ISSN	09740422
Language	English
Publisher	Springer India
Publisher Date	2014-07-15
Publisher Place	India
Access Restriction	Open
Rights License	© Association of Clinical Biochemists of India 2014
Subject Keyword	Curcumin Murine macrophage-like cells IL-18 Necrosis
Content Type	Text
Resource Type	Article
Subject	Clinical Biochemistry

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