miR-136 modulates TGF-β1-induced proliferation arrest by targeting PPP2R2A in keratinocytes.

Content Provider	Europe PMC
Author	Zhang, Dianbao Wang, Jing Wang, Zhe Zhang, Tao Shi, Ping Wang, Xiliang Zhao, Feng Liu, Xiaoyu Lin, Xuewen Pang, Xining
Copyright Year	2015
Abstract	Keratinocytes proliferation is critical for the capacity to heal wounds and accumulating evidences have proved that dysregulation of microRNAs is involved in proliferation of keratinocytes. However, the molecular mechanisms remain to be completely elucidated. Here, we show that miR-136 was significantly decreased by TGF-β1 treatment in HaCaT cells and normal human epidermal keratinocytes (NHEK), and it was a Smad3-dependent manner. By cell proliferation assay and cell cycle analysis, we found that reintroduction of miR-136 by transfection, as well as PPP2R2A silencing, counteracted TGF-β-induced proliferation arrest in HaCaT cells. Further, PPP2R2A was verified as a direct target of miR-136 by dual-luciferase reporter assays and Western blotting. These data suggest that miR-136 may play an important role during TGF-β1-induced proliferation arrest by targeting PPP2R2A in keratinocytes, which might represent a potential target for improving skin wound healing.
ISSN	23146133
Journal	Biomed Research International
Volume Number	2015
PubMed Central reference number	PMC4310454
PubMed reference number	25654102
e-ISSN	23146141
DOI	10.1155/2015/453518
Language	English
Publisher	Hindawi
Publisher Date	2015-01-14
Access Restriction	Open
Rights License	This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Copyright © 2015 Dianbao Zhang et al.
Content Type	Text
Resource Type	Article
Subject	Immunology and Microbiology Medicine Biochemistry, Genetics and Molecular Biology