Dictyopteris undulata Extract Induces Apoptosis via Induction of Endoplasmic Reticulum Stress in Human Colon Cancer Cells.

Content Provider	Europe PMC
Author	Kang, Kyoung Ah Kim, Joon Ki Jeong, Yong Joo Na, Soo-Young Hyun, Jin Won
Copyright Year	2014
Abstract	Background:Induction of endoplasmic reticulum (ER) stress-mediated apoptosis in cancer cells represents an alternative approach for cancer therapy. The objective of this study was to elucidate whether ethanol extract of the marine brown alga Dictyopteris undulata can induce apoptosis, via ER stress, in human colon adenocarcinoma cells.Methods:Anti-proliferative activity was evaluated by the colony forming assay. ER stress response was evaluated using flow cytometry and confocal imaging after Rhod2 and ER tracker staining. The expression of ER stress-related proteins was assessed by Western blotting.Results:D. undulata extract (DUE) inhibited colony forming ability in SW480 cells. Furthermore, DUE induced characteristic signs of ER stress: mitochondrial Ca2+ overloading, ER staining, expression of ER stress-related proteins, phosphorylation of RNA-dependent protein kinase-like ER kinase and inositol requiring enzyme 1, cleavage of activating transcription factor 6, and induction of the pro-apoptotic factors, CCAAT/enhancer-binding protein-homologous protein (CHOP) and caspase-12. Moreover, down-regulation of CHOP by siCHOP RNA attenuated DUE-induced apoptosis.Conclusions:The ER stress response plays an important role in DUE-induced apoptosis in human colon cancer cells.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC4204170&blobtype=pdf
ISSN	22883649
Journal	Journal of Cancer Prevention [J Cancer Prev]
Volume Number	19
PubMed Central reference number	PMC4204170
Issue Number	2
PubMed reference number	25337580
e-ISSN	22883657
DOI	10.15430/jcp.2014.19.2.118
Language	English
Publisher	Korean Society of Cancer Prevention
Publisher Date	2014-06-01
Access Restriction	Open
Rights License	This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Copyright © 2014 Korean Society of Cancer Prevention
Subject Keyword	Marine brown alga Colon adenocarcinoma cells Apoptosis Endoplasmic reticulum stress
Content Type	Text
Resource Type	Article
Subject	Cancer Research