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Recent advances in in vivo genotoxicity testing: prediction of carcinogenic potential using comet and micronucleus assay in animal models.
| Content Provider | Europe PMC |
|---|---|
| Author | Kang, Seung Hun Kwon, Jee Young Lee, Jong Kwon Seo, Young Rok |
| Copyright Year | 2013 |
| Abstract | Genotoxic events have been known as crucial step in the initiation of cancer. To assess the risk of cancer, genotoxicity assays, including comet, micronucleus (MN), chromosomal aberration, bacterial reverse, and sister chromatid exchange assay, can be performed. Compared with in vitro genotoxicity assay, in vivo genotoxicity assay has been used to verify in vitro assay result and definitely provide biological significance for certain organs or cell types. The comet assay can detect DNA strand breaks as markers of genotoxicity. Methods of the in vivo comet assay have been established by Japanese Center for the Validation of Alternative Methods (JaCVAM) validation studies depending on tissue and sample types. The MN can be initiated by segregation error and lagging acentric chromosome fragment. Methods of the in vivo MN assay have been established by Organization for Economic Co-operation and Development (OECD) test guidelines and many studies. Combining the in vivo comet and MN assay has been regarded as useful methodology for evaluating genetic damage, and it has been used in the assessment of potential carcinogenicity by complementarily presenting two distinct endpoints of the in vivo genotoxicity individual test. Few studies have investigated the quantitative relation between in vivo genotoxicity results and carcinogenicity. Extensive studies emphasizes that positive correlation is detectable. This review summarizes the results of the in vivo comet and MN assays that have investigated the genotoxicity of carcinogens as classified by the International Agency for Research on Cancer (IARC) carcinogenicity database. As a result, these genotoxicity data may provide meaningful information for the assessment of potential carcinogenicity and for implementation in the prevention of cancer. |
| Related Links | https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC4189446&blobtype=pdf |
| ISSN | 22883649 |
| Journal | Journal of Cancer Prevention [J Cancer Prev] |
| Volume Number | 18 |
| PubMed Central reference number | PMC4189446 |
| Issue Number | 4 |
| PubMed reference number | 25337557 |
| e-ISSN | 22883657 |
| DOI | 10.15430/jcp.2013.18.4.277 |
| Language | English |
| Publisher | Korean Society of Cancer Prevention |
| Publisher Date | 2013-12-01 |
| Access Restriction | Open |
| Rights License | This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Copyright © 2013 Korean Society of Cancer Prevention |
| Subject Keyword | In vivo genotoxicity Carcinogenicity Comet assay Micronucleus assay |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cancer Research |
