Effects of acute doxorubicin treatment on hepatic proteome lysine acetylation status and the apoptotic environment.

Content Provider	Europe PMC
Author	Dirks-Naylor, Amie J Kouzi, Samir A Bero, Joseph D Tran, Ngan TK Yang, Sendra Mabolo, Raean
Copyright Year	2014
Abstract	AIM: To determine if doxorubicin (Dox) alters hepatic proteome acetylation status and if acetylation status was associated with an apoptotic environment. METHODS: Doxorubicin (20 mg/kg; Sigma, Saint Louis, MO; n = 8) or NaCl (0.9%; n = 7) was administered as an intraperitoneal injection to male F344 rats, 6-wk of age. Once animals were treated with Dox or saline, all animals were fasted until sacrifice 24 h later. RESULTS: Dox treatment decreased proteome lysine acetylation likely due to a decrease in histone acetyltransferase activity. Proteome deacetylation may likely not be associated with a proapoptotic environment. Dox did not increase caspase-9, -8, or -3 activation nor poly (adenosine diphosphate-ribose) polymerase-1 cleavage. Dox did stimulate caspase-12 activation, however, it likely did not play a role in apoptosis induction. CONCLUSION: Early effects of Dox involve hepatic proteome lysine deacetylation and caspase-12 activation under these experimental conditions.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC4160530&blobtype=pdf
ISSN	19498454
Volume Number	5
DOI	10.4331/wjbc.v5.i3.377
PubMed Central reference number	PMC4160530
Issue Number	3
PubMed reference number	25225604
Journal	World Journal of Biological Chemistry [World J Biol Chem]
e-ISSN	19498454
Language	English
Publisher	Baishideng Publishing Group Inc
Publisher Date	2014-08-01
Access Restriction	Open
Rights License	©2014 Baishideng Publishing Group Inc. All rights reserved.
Subject Keyword	Sirtuin 1 Sirtuin 3 Caspase Apoptosis Acetylation Histone deacetylase Histone acetyltransferase
Content Type	Text
Resource Type	Article
Subject	Biochemistry (medical)