Neuronal-like cell differentiation of non-adherent bone marrow cell-derived mesenchymal stem cells.

Content Provider	Europe PMC
Author	Wu, Yuxin Zhang, Jinghan Ben, Xiaoming
Copyright Year	2013
Abstract	Non-adherent bone marrow cell-derived mesenchymal stem cells from C57BL/6J mice were separated and cultured using the “pour-off” method. Non-adherent bone marrow cell-derived mesenchymal stem cells developed colony-forming unit-fibroblasts, and could be expanded by supplementation with epidermal growth factor. Immunocytochemistry showed that the non-adherent bone marrow cell-derived mesenchymal stem cells exposed to basic fibroblast growth factor/epidermal growth factor/nerve growth factor expressed the neuron specific markers, neurofilament-200 and NeuN, in vitro. Non-adherent bone marrow cell-derived mesenchymal stem cells from β-galactosidase transgenic mice were also transplanted into focal ischemic brain (right corpus striatum) of C57BL/6J mice. At 8 weeks, cells positive for LacZ and β-galactosidase staining were observed in the ischemic tissues, and cells co-labeled with both β-galactosidase and NeuN were seen by double immunohistochemical staining. These findings suggest that the non-adherent bone marrow cell-derived mesenchymal stem cells could differentiate into neuronal-like cells in vitro and in vivo.
ISSN	16735374
Volume Number	8
PubMed Central reference number	PMC4146062
Issue Number	22
PubMed reference number	25206516
Journal	Neural Regeneration Research [Neural Regen Res]
e-ISSN	18767958
DOI	10.3969/j.issn.1673-5374.2013.22.007
Language	English
Publisher	Medknow Publications & Media Pvt Ltd
Publisher Date	2013-08-01
Publisher Place	India
Access Restriction	Open
Rights License	This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Copyright: © Neural Regeneration Research
Subject Keyword	neural regeneration stem cells non-adherent bone marrow cell-derived mesenchymal stem cells neuronal-like cells colony-forming unit-fibroblasts proliferation differentiation beta-galactosidase transgenic mouse cell transplantation cerebral ischemia bone marrow cells-derived mesenchymal stem cells grants-supported paper neuroregeneration
Content Type	Text
Resource Type	Article
Subject	Developmental Neuroscience Neuroscience